Publication detail

Comparison of Voltage-Sensitive Dye di-4-ANNEPS Effects in Isolated Hearts of Rat, Guinea Pig, and Rabbit

FIALOVÁ, K. KOLÁŘOVÁ, J. PROVAZNÍK, I. NOVÁKOVÁ, M.

Original Title

Comparison of Voltage-Sensitive Dye di-4-ANNEPS Effects in Isolated Hearts of Rat, Guinea Pig, and Rabbit

English Title

Comparison of Voltage-Sensitive Dye di-4-ANNEPS Effects in Isolated Hearts of Rat, Guinea Pig, and Rabbit

Type

conference paper

Language

Czech

Original Abstract

Voltage-sensitive dyes (VSDs) are used for recording of monophasic action potentials (MAPs) by optical method in excitable tissues. Their direct effects on the tissue are not completely elucidated. Previously we studied effects of VSD di-4-ANEPPS during staining and washout in Langendorff-perfused guinea pig and rabbit isolated hearts. However, most often used species in basic cardiology is rat. Therefore, we decided to compare the electrophysiological effects of VSD in isolated hearts of rabbit, guinea pig, and rat. Touch-less electrogram was recorded, the heart rate assessed and normalized. The type and incidence of arrhythmias were evaluated. Based on our results - although the procedure of staining with the dye affects electrophysiological properties of the myocardium in all studied species - rat heart may be considered as the most suitable for electrophysiological studies using di-4-ANEPPS.

English abstract

Voltage-sensitive dyes (VSDs) are used for recording of monophasic action potentials (MAPs) by optical method in excitable tissues. Their direct effects on the tissue are not completely elucidated. Previously we studied effects of VSD di-4-ANEPPS during staining and washout in Langendorff-perfused guinea pig and rabbit isolated hearts. However, most often used species in basic cardiology is rat. Therefore, we decided to compare the electrophysiological effects of VSD in isolated hearts of rabbit, guinea pig, and rat. Touch-less electrogram was recorded, the heart rate assessed and normalized. The type and incidence of arrhythmias were evaluated. Based on our results - although the procedure of staining with the dye affects electrophysiological properties of the myocardium in all studied species - rat heart may be considered as the most suitable for electrophysiological studies using di-4-ANEPPS.

Keywords

Voltage-sensitive dyes, monophasic action potential, perfused heart

Key words in English

Voltage-sensitive dyes, monophasic action potential, perfused heart

Authors

FIALOVÁ, K.; KOLÁŘOVÁ, J.; PROVAZNÍK, I.; NOVÁKOVÁ, M.

Released

26. 9. 2010

Publisher

IEEE

Location

Belfast, Ireland

ISBN

978-1-4244-7319-9

Book

Computing in Cardiology

ISBN

0276-6574

Periodical

Computers in Cardiology

State

United States of America

Pages from

565

Pages to

568

Pages count

4

BibTex

@inproceedings{BUT34993,
  author="Kateřina {Fialová} and Jana {Kolářová} and Valentine {Provazník} and Marie {Nováková}",
  title="Comparison of Voltage-Sensitive Dye di-4-ANNEPS Effects in Isolated Hearts of Rat, Guinea Pig, and Rabbit",
  booktitle="Computing in Cardiology",
  year="2010",
  journal="Computers in Cardiology",
  pages="565--568",
  publisher="IEEE",
  address="Belfast, Ireland",
  isbn="978-1-4244-7319-9",
  issn="0276-6574"
}