Publication detail

Parallel-Mode Confocal Microscopy

CHMELÍK, R., HARNA, Z.

Original Title

Parallel-Mode Confocal Microscopy

Type

journal article - other

Language

English

Original Abstract

Conventional confocal microscopes are based on the dual scanning of a specimen by the images of a point source and of a point detector. Hence, their imaging mode is serial, i.e., the confocal image is obtained from the sequence of single-point or multiple-point partial images. Parallel-mode confocal imaging is possible on the basis of broad-source image-plane holography. We adapted this classical-holography technique for real-time reflected-light microscopy. The imaging speed of the parallel-mode confocal microscope is not limited by any part of the optical system, but only by the image detection and storage systems. Both the image phase and the image amplitude are reconstructed from the interference signal. It was verified both theoretically and experimentally that the main imaging parameters of the microscope are comparable with those of a conventional confocal microscope. The only exception is the imaging speed, which can be much higher.

Keywords

Confocal microscopy, Holographic applications, Real-time imaging.

Authors

CHMELÍK, R., HARNA, Z.

RIV year

2001

Released

1. 1. 1999

ISBN

0091-3286

Periodical

Optical Engineering

Year of study

38

Number

10

State

United States of America

Pages from

1635

Pages to

1639

Pages count

5

BibTex

@article{BUT39686,
  author="Radim {Chmelík} and Zdeněk {Harna}",
  title="Parallel-Mode Confocal Microscopy",
  journal="Optical Engineering",
  year="1999",
  volume="38",
  number="10",
  pages="5",
  issn="0091-3286"
}