Publication detail

Production and biochemical characterization of polygalacturonases produced by Aureobasidium pullulans from forest soil

OMELKOVÁ, J.

Original Title

Production and biochemical characterization of polygalacturonases produced by Aureobasidium pullulans from forest soil

Type

journal article - other

Language

English

Original Abstract

The production of individual form of extracellular polygalacturonase by Aureobasidium pullulans from forest soil was found to depend on the pH of cultivation medium as well as on the nitrogen source in the precultivation or cultivation medium. Polygalacturonases were purified and characterized. The pH optima of polygalacturonases produced in the first phases of cultivation (24 or 48 h) and after 10 days as well as their molecular masses, isoelectric points, action pattern and ability to cleave polymeric and oligomeric substrates were different. Generally, polygalacturonases with random action pattern (EC 3.2.1.15) were produced only in the first phases of cultivation in acidic medium. The function of these enzymes for A. pullulans in the colonization of plant material rather than in the destruction of plant was hypothesized in physiological conditions. Exopolygalacturonases (EC 3.2.1.67) with terminal action pattern were produced in later phases of growth. Oligogalacturonate hydrolase as well as strongly basic polygalacturonase with unusual action pattern on substrates were found.

Keywords

Aureobasidium pullulans; exopolygalacturonase; multiple forms; oligogalacturonate hydrolase; polygalacturonase

Authors

OMELKOVÁ, J.

RIV year

2006

Released

19. 1. 2006

ISBN

1590-4261

Edition number

ANNALS OF MICROBIOLO

ISBN

1590-4261

Periodical

Annals of Microbiology

Year of study

56

Number

1

State

Republic of Italy

Pages from

35

Pages to

40

Pages count

6

BibTex

@article{BUT43211,
  author="Jiřina {Omelková}",
  title="Production and biochemical characterization of polygalacturonases produced by Aureobasidium pullulans from forest soil",
  journal="Annals of Microbiology",
  year="2006",
  volume="56",
  number="1",
  pages="35--40",
  issn="1590-4261"
}