Publication detail

Comparison of two different solvents employed for pressurised fluid extraction of stevioside from Stevia rebaudiana : methanol versus water

PÓL, J. VARAĎOVÁ OSTRÁ, E. KARÁSEK, P. ROTH, M. BENEŠOVÁ, K. KOTLAŘÍKOVÁ, P. ČÁSLAVSKÝ, J.

Original Title

Comparison of two different solvents employed for pressurised fluid extraction of stevioside from Stevia rebaudiana : methanol versus water

Type

journal article - other

Language

English

Original Abstract

Pressurised fluid extraction using water or methanol was employed for the extraction of stevioside from Stevia rebaudiana Bertoni. The extraction method was optimised in terms of temperature and duration of the static or the dynamic step. Extracts were analysed by liquid chromatography followed by UV and mass-spectrometric (MS) detections. Thermal degradation of stevioside was the same in both solvents within the range 70 to 160 deg. C. Methanol showed better extraction ability for isolation of stevioside from Stevia rebaudiana leaves than water within the range 110 to 160 deg. C. However, water represents the green alternative to methanol. The limit of detection of stevioside in the extract analysed was 30 ng for UV detection and 2 ng for MS detection.

Keywords

Pressurised fluid extraction; Liquid chromatography / Mass spectrometry; Stevia rebaudiana; Stevioside

Authors

PÓL, J.; VARAĎOVÁ OSTRÁ, E.; KARÁSEK, P.; ROTH, M.; BENEŠOVÁ, K.; KOTLAŘÍKOVÁ, P.; ČÁSLAVSKÝ, J.

RIV year

2007

Released

15. 8. 2007

Publisher

Springer

Location

Berlin - Heidelberg

ISBN

1618-2642

Periodical

Analytical and Bioanalytical Chemistry

Year of study

388

Number

8

State

Federal Republic of Germany

Pages from

1847

Pages to

1857

Pages count

11

BibTex

@article{BUT43961,
  author="Jaroslav {Pól} and Elena {Ostrá} and Pavel {Karásek} and Michal {Roth} and Karolína {Benešová} and Pavla {Kožušníková} and Josef {Čáslavský}",
  title="Comparison of two different solvents employed for pressurised fluid extraction of stevioside from Stevia rebaudiana : methanol versus water",
  journal="Analytical and Bioanalytical Chemistry",
  year="2007",
  volume="388",
  number="8",
  pages="1847--1857",
  issn="1618-2642"
}