Publication result detail

Comparison of biosensoric and chromatographic methods for the detection of pesticides

Zuzana Grosmanova, Jan Krejci, Jaroslav Tynek, Petr Cuhra a Sona Barsova

Original Title

Comparison of biosensoric and chromatographic methods for the detection of pesticides

English Title

Comparison of biosensoric and chromatographic methods for the detection of pesticides

Type

Peer-reviewed article not indexed in WoS or Scopus

Original Abstract

Screen-printed biosensors with immobilized acetylcholine esterase (AChE) were used for measuring fruit and vegetable samples that had first been analysed using gas and highperformance liquid chromatography. The output signal for the biosensors is the current, which is used to calculate relative inhibition (RI), a measured quantity. RI is proportional to the inhibiting (toxic) effect of organophosphates and carbamates. Measurements with AChE biosensors are not easily reproducible. This problem is solved by the choice of an arbitrary toxicity standard of 1.25 uM Syntostigmin. Measurements were evaluated by the ratio of the relative inhibition of the sample against the relative inhibition of Syntostigmin. Results obtained from the biosensor match those of chromatography in 19 out of 38 total measurements made and for nine out of 19 positive samples. The confirmation rate was 50%. Future work must check the limit of 0.1 and the independent control of inhibiting pesticides contents after measurements using the biosensor.

English abstract

Screen-printed biosensors with immobilized acetylcholine esterase (AChE) were used for measuring fruit and vegetable samples that had first been analysed using gas and highperformance liquid chromatography. The output signal for the biosensors is the current, which is used to calculate relative inhibition (RI), a measured quantity. RI is proportional to the inhibiting (toxic) effect of organophosphates and carbamates. Measurements with AChE biosensors are not easily reproducible. This problem is solved by the choice of an arbitrary toxicity standard of 1.25 uM Syntostigmin. Measurements were evaluated by the ratio of the relative inhibition of the sample against the relative inhibition of Syntostigmin. Results obtained from the biosensor match those of chromatography in 19 out of 38 total measurements made and for nine out of 19 positive samples. The confirmation rate was 50%. Future work must check the limit of 0.1 and the independent control of inhibiting pesticides contents after measurements using the biosensor.

Keywords

Acetylcholine esterase; Food analysis; Enzyme-based biosensor

Key words in English

Acetylcholine esterase; Food analysis; Enzyme-based biosensor

Authors

Zuzana Grosmanova, Jan Krejci, Jaroslav Tynek, Petr Cuhra a Sona Barsova

RIV year

2010

Released

15.10.2005

Publisher

Taylor & Francis

Volume

85

Number

12-13

Pages from

885

Pages count

8

BibTex

@article{BUT45537,
  author="Zuzana {Grosmanová} and Jan {Krejčí} and Jaroslav {Týnek}",
  title="Comparison of biosensoric and chromatographic methods for the detection of pesticides",
  year="2005",
  volume="85",
  number="12-13",
  pages="8"
}