Publication detail

Polygalacturonases from potato tubers

OMELKOVÁ, J.

Original Title

Polygalacturonases from potato tubers

Type

journal article - other

Language

English

Original Abstract

Potato tubers produce small amount of polygalacturonase. Multiple forms of this enzyme were observed with isoelectric points between 4.6 and 7.2 (major form about 6.5). Polygalacturonase isoforms were characterized by their molecular masses, pH optima, optimum of temperature, thermal stability, KM on polymeric substrate and action pattern on polymeric and oligomeric substrate. All forms showed highly random cleavage pattern of pectate and absence of activity towards both di(D-galactosiduronic) acid and tri(D-galactosiduronic) acid, particularly the trimer. Based on these results potato polygalacturonase (endopolygalacturonase, EC 3.2.1.15) can be characterized as an enzyme with unusual degradation of oligomeric substrates yielding trimer as the end product.

Keywords

OLIGOGALACTURONIC ACIDS; ENDOPOLYGALACTURONASE-I; ASPERGILLUS-NIGER; CRYSTAL-STRUCTURE; TOMATO FRUIT; PURIFICATION; PECTINESTERASE; FIRMNESS; ENZYMES; WEEVIL

Authors

OMELKOVÁ, J.

RIV year

2005

Released

12. 5. 2005

ISBN

0006-3088

Periodical

Biológia

Year of study

2005(3)

Number

60

State

United States of America

Pages from

267

Pages to

273

Pages count

6

BibTex

@article{BUT46092,
  author="Jiřina {Omelková}",
  title="Polygalacturonases from potato tubers",
  journal="Biológia",
  year="2005",
  volume="2005(3)",
  number="60",
  pages="267--273",
  issn="0006-3088"
}