Publication detail

Identification of viable Lactobacillus cells in fermented dairy products

TRACHTOVÁ, Š. ŠPANOVÁ, A. RITTICH, B.

Original Title

Identification of viable Lactobacillus cells in fermented dairy products

Type

journal article - other

Language

English

Original Abstract

The combination PCR and ethidium monoazide (EMA-PCR) is a new metod for selective distinction between viable and dead bacterial cells. The general application of EMA is based on penetration into 'dead' cells with compromised membráně (cell wall systems) integrity. DNA bound to EMA cannot be PCR amplified. Live cells remain intact and only DNA from viable cells can be amplified. Experiments were performed using probiotic bacterial strain Lactobacillus paracasei subsp. paracasei CCDM 211/06 of human origin. The experimental conditions (time of cell incubation with EMA, and cell irradiation) were optimized. In the next step instead for live-dead cell distinction propidium monoazide was used. The Lactobacillus species specific primers were used for DNA amplification. The PCR products (250 bp) were separated using electrophoresis in 1.8 % agarose gel. The method was applied for live and dead cells identification in milk product (yoghurt).

Keywords

ethidium monoazide, viable and dead bacterial cells

Authors

TRACHTOVÁ, Š.; ŠPANOVÁ, A.; RITTICH, B.

RIV year

2008

Released

9. 9. 2008

Publisher

Chemické listy

Location

Brno

ISBN

1213-7103

Periodical

Chemické listy

Year of study

102

Number

15

State

Czech Republic

Pages from

805

Pages to

806

Pages count

2

BibTex

@article{BUT46735,
  author="Štěpánka {Trachtová} and Alena {Španová} and Bohuslav {Rittich}",
  title="Identification of viable Lactobacillus cells in fermented dairy products",
  journal="Chemické listy",
  year="2008",
  volume="102",
  number="15",
  pages="805--806",
  issn="1213-7103"
}