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KUBÁŇ, P. PELCOVÁ, P. KUBÁŇ, V. KLAKURKOVÁ, L. PURNENDU K., D.
Original Title
Gravity-flow open tubular cation chromatography
Type
journal article - other
Language
English
Original Abstract
We describe ion chromatography (IC) on open tubular cation exchange columns with a controllable capacity multilayered stationary phase architecture. The columns of relatively large bore (75 lm id) are fabricated by coating fused-silica capillaries with multiple layers of poly(butadiene-maleic acid) (PBMA) copolymer and crosslinking the deposited layers by thermally initiated radical polymerisation. Column capacity increases in a predictable manner with increase in the number of successively coated layers. Gravity flow with a modest head (a2 m) can provide the desired separations within a reasonable period. We provide a minimalist configuration where no suppression is used, the sample is injected hydrodynamically as in CE, and detection is accomplished by an inexpensive homebuilt contactless conductivity detector or a capacitance to voltage digital converter. A 1 m long 75 lm bore column coated with two layers of PBMA allows gravity-flow open tubular IC to separate four alkali cations in a10 min with a 1 mM tartaric acid (TA) eluent. Simultaneous separation of alkali and alkaline earth metal cations can be accomplished in less than 25 min using 1.75 mM pyridinedicarboxylic acid as an eluent. Contactless conductometric detection (C4D) allows LODs down to 150 nmol/L, corresponding to 30 fmol injections. Analysis of real water samples is demonstrated.
Keywords
Cations ; Contactless conductometric detection; Gravity-flow; Open tubular ion chromatography
Authors
KUBÁŇ, P.; PELCOVÁ, P.; KUBÁŇ, V.; KLAKURKOVÁ, L.; PURNENDU K., D.
RIV year
2008
Released
20. 8. 2008
Publisher
WILEY-VCH Verlag GmbH&Co. KGaA
Location
Weinheim, DE
ISBN
1615-9306
Periodical
Journal of Separation Science
Year of study
Number
31
State
Federal Republic of Germany
Pages from
2745
Pages to
2753
Pages count
9
BibTex
@article{BUT47036, author="Petr {Kubáň} and Pavlína {Pelcová} and Vlastimil {Kubáň} and Lenka {Klakurková} and Dasgupta {Purnendu K.}", title="Gravity-flow open tubular cation chromatography", journal="Journal of Separation Science", year="2008", volume="2008", number="31", pages="2745--2753", issn="1615-9306" }