Experimental methods for simultaneous measurement of action potentials and electrograms in isolated heart

journal article in Web of Science

English

Monophasic action potential (MAP) can be recorded from the heart surface by optical method based on fluorescence measurement. The motion of isolated heart during experiment caused additional noise in recorded signal. The motion artifact can be eliminated by ratiometric fluorescence emission measurements. This study is based on experiments in which optical MAP measurement is done by single-wavelength and dual-wavelength measurement of fluorescence emission. Both recording setups are presented and their advantages and disadvantages are discussed. MAPs recorded by both methods from isolated rabbit hearts perfused according to Langendorff are presented. Simultaneous electrograms (EG) and MAPs recording are analyzed and measurement of velocity of impulse conduction through heart tissue is presented.

isolated heart, action potential, electrogram, voltage sensitive dye, ratiometry

KOLÁŘOVÁ, J.; FIALOVÁ, K.; JANOUŠEK, O.; NOVÁKOVÁ, M.; PROVAZNÍK, I.

2010

28. 5. 2010

AV ČR

Praha

0862-8408

Physiological Research

59

Suppl 1

Czech Republic

S71

S80

10