Publication detail

Monolayers of photosystem II on gold electrodes with enhanced sensor response - effect of porosity and protein layer arrangement

KREJČÍ, J. MALY, J. JAKUBKA, L. ŠTEFFAN, P. MASOJÍDEK, J.

Original Title

Monolayers of photosystem II on gold electrodes with enhanced sensor response - effect of porosity and protein layer arrangement

Type

journal article in Web of Science

Language

English

Original Abstract

Mass transport of the bulk of the analyte to the electrode and through the bioactive layer can be significantly improved by use of the nanoelectrode array and defined arrangement of protein film. This phenomenon has been studied by (i) atomic-force microscopy, (ii) electrochemical measurements of PSII activity, and (iii) digital simulations for an oriented monolayer of histidine-tagged photosystem II (PSII) immobilized on nitrilotriacetic acid (NTA)-modified gold electrodes. The output signal of the electrochemical biosensor is controlled by (i) mass transport from the bioactive layer to electrode and (ii) mass transport between the bulk of the analyte and the electrode. Mass transport through the bioactive layer was electrochemically studied for PSII self-assembled on gold screen-printed electrodes. A densely packed monolayer of PSII has a significant shielding effect toward the diffusion of redox mediator duroquinone (DQ). Mass transport to the planar electrode surface was improved by co-immobilization of bovine-serum albumin (BSA) as spacer biomolecule in the monolayer of PSII. Correlation between the electrochemical properties and surface arrangement of the resulting protein films was clearly observable and confirmed the improved mass-transport properties of structured enzyme monolayers. On the basis of this observation, the application of a bottom-up approach for improvement of electrode performance was proposed and digitally simulated for an infinite array of electrodes ranging in diameter from 50 nm to 5 mu m. The nanoelectrode array, with the optimum time window selected for measurements, enables enhancement of mass transport between the bulk of the analyte and the macroelectrode by a factor of up to 50 in comparison with "classical" planar electrodes. Use of a time window enables minimization of crosstalk between individual electrodes in the array. The measurements require methods which suppress the double-layer capacity.

Keywords

protein monolayer; photosystem II; nanostructured electrodes; biosensors; mass transport

Authors

KREJČÍ, J.; MALY, J.; JAKUBKA, L.; ŠTEFFAN, P.; MASOJÍDEK, J.

RIV year

2005

Released

15. 4. 2005

Publisher

SPRINGER HEIDELBERG

Location

HEIDELBERG, GERMANY

ISBN

1618-2642

Periodical

Analytical and Bioanalytical Chemistry

Year of study

381

Number

8

State

Federal Republic of Germany

Pages from

1558

Pages to

1567

Pages count

9

BibTex

@article{BUT48435,
  author="Jan {Krejčí} and Jiri {Maly} and Luboš {Jakubka} and Pavel {Šteffan} and Jiří {Masojídek}",
  title="Monolayers of photosystem II on gold electrodes with enhanced sensor response - effect of porosity and protein layer arrangement",
  journal="Analytical and Bioanalytical Chemistry",
  year="2005",
  volume="381",
  number="8",
  pages="1558--1567",
  issn="1618-2642"
}