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ADAM, V. MIKELOVÁ, R. HUBÁLEK, J. HANUSTIAK, P. BEKLOVÁ, M. HORNA, A. TRNKOVÁ, L. ZEMAN, L. KIZEK, R.
Original Title
Utilizing of square wave voltammetry to detect flavonoids in the presence of human urine
Type
journal article - other
Language
English
Original Abstract
About biological affecting of flavonoids on animal organisms is known less, thus we selected flavonoids, flavanones and flavones, and their glycosides, which were examined as potential inducers of cytochrome(s) P450 when administrated by gavages into experimental male rats. The study was focused on induction of CYP1A1, the major cytochrome P450 involved in carcinogen activation. The data obtained demonstrate the necessity of taking into account not only ability of flavonoids to bind to Ah receptor (induction factor) but also to concentrate on their distribution and metabolism (including colon microflora) in the body. After that we examined certain flavonoids as potential inducers of cytochrome P450, we wanted to suggest and optimize suitable electrochemical technique for determination of selected flavonoids (quercetin, quercitrin, rutin, chrysin and diosmin) in body liquids. For these purposes, we selected square wave voltannetry using carbon paste electrode.
Keywords
flavonoids; antioxidant; carbon paste electrode; square wave voltammetry; western blot analysis; cytochrome P450; cancer
Authors
ADAM, V.; MIKELOVÁ, R.; HUBÁLEK, J.; HANUSTIAK, P.; BEKLOVÁ, M.; HORNA, A.; TRNKOVÁ, L.; ZEMAN, L.; KIZEK, R.
Released
15. 10. 2007
Publisher
MDPI
Location
Basel, Švýcarsko
ISBN
1424-8220
Periodical
SENSORS
Year of study
7
Number
10
State
Swiss Confederation
Pages from
2402
Pages to
2418
Pages count
17
URL
http://www.mdpi.com/1424-8220/7/10/2402
Full text in the Digital Library
http://hdl.handle.net/11012/70075
BibTex
@article{BUT49680, author="ADAM, V. and MIKELOVÁ, R. and HUBÁLEK, J. and HANUSTIAK, P. and BEKLOVÁ, M. and HORNA, A. and TRNKOVÁ, L. and ZEMAN, L. and KIZEK, R.", title="Utilizing of square wave voltammetry to detect flavonoids in the presence of human urine", journal="SENSORS", year="2007", volume="7", number="10", pages="2402--2418", doi="10.3390/s7102402", issn="1424-8220", url="http://www.mdpi.com/1424-8220/7/10/2402" }