Publication detail

Cell toxicity and preparation of streptavidin-modified iron nanoparticles and glutathione-modified cadmium-based quantum dots

PEKÁRKOVÁ, J. DRBOHLAVOVÁ, J. BABULA, P. ADAM, V. HUBÁLEK, J. PROVAZNÍK, I. KIZEK, R.

Original Title

Cell toxicity and preparation of streptavidin-modified iron nanoparticles and glutathione-modified cadmium-based quantum dots

Type

journal article - other

Language

English

Original Abstract

This paper is focused on the investigation of conjugate nanostructures consisting of superparamagnetic nanoparticles (NPs) and CdSe/CdS quantum dots (QDs) with core/shell structure. In the first step, we prepared streptavidin (STP) conjugated Fe2O3 magnetic particles. The next stage was preparation of QDs using method based on surface modification of hydrophobic core/shell CdSe/CdS with biotinylated glutathione. Glutathione (GSH) belongs to the most abundant peptides in organisms maintaining redox status, which, from the particle point of view, makes the QDs water-soluble and stabilized in aqueous solution. Biotin molecule is known for its excellent affinity to streptavidin and hence allows the conjugation of both nanostructures (magnetic and light-emitting). We tested the influence of both prepared nanostructures on growth and viability of fibroblasts and BY-2 tobacco cells, respectively. NPs-STP and QDs-GSH may not pose a threat to cells, but non-modified nanoparticles and QDs without GSH had a very adverse effect on cells.

Keywords

Quantum dots; Magnetic nanoparticles; Cell toxicity

Authors

PEKÁRKOVÁ, J.; DRBOHLAVOVÁ, J.; BABULA, P.; ADAM, V.; HUBÁLEK, J.; PROVAZNÍK, I.; KIZEK, R.

RIV year

2010

Released

5. 9. 2010

Publisher

Elsevier Ltd

Location

Linz, Austria

ISBN

1877-7058

Periodical

Procedia Engineering

Year of study

2010

Number

5

State

Kingdom of the Netherlands

Pages from

922

Pages to

925

Pages count

4

BibTex

@article{BUT50993,
  author="Jana {Pekárková} and Jana {Drbohlavová} and Petr {Babula} and Vojtěch {Adam} and Jaromír {Hubálek} and Valentine {Provazník} and René {Kizek}",
  title="Cell toxicity and preparation of streptavidin-modified iron nanoparticles and glutathione-modified cadmium-based quantum dots",
  journal="Procedia Engineering",
  year="2010",
  volume="2010",
  number="5",
  pages="922--925",
  issn="1877-7058"
}