Analysis of casein content in cow milk

Analysis of casein content in cow milk

conference paper

Czech

Milk includes huge group of albumins - caseins. Caseins play crucial role in cheese production. They influence cheese ageing and quality. Besides they have significant emulsifying, nutrition and bonds-production effects and they are, therefore, often used in food industry. Caseins are principal by various fractions different in properties, function and their using. Therefore, these fractions are monitored and we developed simple method on assesment most important fraction in single analysis. Method was applied to complex experiment monitoring changes substitution particular fraction depending on constitution feeding mixture. For determination, capillary zone electrophoresis (CZE) was used employing non-coated silica capillary I.D. 50 mikrom, total size 125 cm, effective size 100 cm. Separation voltage was set at 30 kV using basic electrolyte 23 mM phosphate buffer with 8 M urea. UV-VIS detector was used following at 214 nm. Analyzed was defatted freeze-dried milk after solubilization in reducing TRIS buffer with DTT and urea. Time of analyses was 2 o'clock. In order to reduce time of analysis and achieve higher affectivity and better separation method parameters were optimized.

Milk includes huge group of albumins - caseins. Caseins play crucial role in cheese production. They influence cheese ageing and quality. Besides they have significant emulsifying, nutrition and bonds-production effects and they are, therefore, often used in food industry. Caseins are principal by various fractions different in properties, function and their using. Therefore, these fractions are monitored and we developed simple method on assesment most important fraction in single analysis. Method was applied to complex experiment monitoring changes substitution particular fraction depending on constitution feeding mixture. For determination, capillary zone electrophoresis (CZE) was used employing non-coated silica capillary I.D. 50 mikrom, total size 125 cm, effective size 100 cm. Separation voltage was set at 30 kV using basic electrolyte 23 mM phosphate buffer with 8 M urea. UV-VIS detector was used following at 214 nm. Analyzed was defatted freeze-dried milk after solubilization in reducing TRIS buffer with DTT and urea. Time of analyses was 2 o'clock. In order to reduce time of analysis and achieve higher affectivity and better separation method parameters were optimized.

milk, milk albumin, casein, CZE, capillary zone electrophoresis, constitution pasturage

milk, milk albumin, casein, CZE, capillary zone electrophoresis, constitution pasturage

DVOŘÁK, M.; VESPALCOVÁ, M.; RITTICH, B.

3. 12. 2010

Vysoké učení technické v Brně, Fakulta Chemická

Purkyňova 464/118, 612 00 Brno

978-80-214-4212-2

Studentská odborná konference Chemie a společnost 2010 - Sborník příspěvků

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