Přístupnostní navigace
E-application
Search Search Close
Publication detail
HYNEK, D. PRÁŠEK, J. KOUDELKA, P. CHOMOUCKÁ, J. TRNKOVÁ, L. ADAM, V. HUBÁLEK, J. KIZEK, R.
Original Title
Advantages and progress in the analysis of DNA by using mercury and amalgam electrodes - Review
Type
journal article - other
Language
English
Original Abstract
The electroactivity of DNA was discovered in 1958 by Emil Palecek. Since than, there have been done great progress and development in electrochemistry of nucleic acids at various electrodes. In this review, after brief overview of milestones in research in electrochemistry, history of electroanalysis of DNA follows. Then, the attention is aimed at various electrochemical methods using a mercury electrode as a working one including linear sweep and cyclic polarography/voltammetry (elimination polarography/voltammetry), differential pulse polarography/voltammetry, square wave polarography/voltammetry, AC polarography/voltammetry and chronopotentiometry for analysis of DNA. Coupling of adsorptive transfer stripping technique to the above-mentioned methods is very promising for nucleic acid studying and is discussed. Further, advantages of mercury and amalgam electrodes are mentioned. The advantages of coupling of electroanalysis of DNA at mercury electrodes with paramagnetic particles based isolation of target molecules are shown.
Keywords
DNA, mercury electrode, DNA hybridization, nucleic acid, reduction of nucleobases, genosensors, biosensors, nanomaterials, magnetic beads, nanotechnology, automation
Authors
HYNEK, D.; PRÁŠEK, J.; KOUDELKA, P.; CHOMOUCKÁ, J.; TRNKOVÁ, L.; ADAM, V.; HUBÁLEK, J.; KIZEK, R.
RIV year
2011
Released
24. 5. 2011
Publisher
Bentham Science Publishers Ltd.
ISBN
1877-9476
Periodical
Current Physical Chemistry
Year of study
1
Number
4
State
Kingdom of the Netherlands
Pages from
299
Pages to
324
Pages count
26
BibTex
@article{BUT72550, author="HYNEK, D. and PRÁŠEK, J. and KOUDELKA, P. and CHOMOUCKÁ, J. and TRNKOVÁ, L. and ADAM, V. and HUBÁLEK, J. and KIZEK, R.", title="Advantages and progress in the analysis of DNA by using mercury and amalgam electrodes - Review", journal="Current Physical Chemistry", year="2011", volume="1", number="4", pages="299--324", issn="1877-9476" }