Publication detail

Electrophoretic fingerprint metallothionein analysis as a potential prostate cancer biomarker

KŘÍŽKOVÁ, S. RYVOLOVÁ, M. GUMULEC, J. MASAŘÍK, M. ADAM, V. MAJZLÍK, P. HUBÁLEK, J. PROVAZNÍK, I. KIZEK, R.

Original Title

Electrophoretic fingerprint metallothionein analysis as a potential prostate cancer biomarker

Type

journal article - other

Language

English

Original Abstract

Prostate-specific antigen (PSA) is a routinely used marker of prostate cancer; however, the cut-off values for unambiguous positive/negative prostate cancer diagnoses are not defined. Therefore, despite the best effort, certain percentage of misdiagnosed cases is being recorded every year. For this reason, search formore specific diagnosticmarkers is of great interest. In this study, systematic comparison of PSA and metallothionein (MT) levels in blood serum of 46 prostate cancer-diagnosed patients is presented. It is clearly demonstrated that PSA levels vary significantly and despite normal total PSA values in the range of 0 - 4 ng/mL were obtained in over 36.9% of cases, positive prostate cancer was diagnosed by biopsy. In contrary, MT levels were considerably elevated in all tested samples and no significant variations were observed. These results are indicating the potential of MT as an additional prostate cancer marker reducing, in combination with PSA, the probability of false positive/negative diagnosis. To increase the throughput of the screening, chip-based capillary electrophoresis was suggested as a rapid and effective method for the fingerprinting analysis of prostate cancer from diseased blood sera.

Keywords

Blood marker, Glutathione, Metallothionein, Prostate cancer, Prostate-specific antigen

Authors

KŘÍŽKOVÁ, S.; RYVOLOVÁ, M.; GUMULEC, J.; MASAŘÍK, M.; ADAM, V.; MAJZLÍK, P.; HUBÁLEK, J.; PROVAZNÍK, I.; KIZEK, R.

RIV year

2011

Released

25. 7. 2011

ISBN

0173-0835

Periodical

Electrophoresis

Year of study

2011

Number

32

State

Federal Republic of Germany

Pages from

1952

Pages to

1961

Pages count

10

BibTex

@article{BUT72631,
  author="Soňa {Křížková} and Markéta {Vaculovičová} and Jaromír {Gumulec} and Michal {Masařík} and Vojtěch {Adam} and Petr {Majzlík} and Jaromír {Hubálek} and Valentine {Provazník} and René {Kizek}",
  title="Electrophoretic fingerprint metallothionein analysis as a potential prostate cancer biomarker",
  journal="Electrophoresis",
  year="2011",
  volume="2011",
  number="32",
  pages="1952--1961",
  issn="0173-0835"
}