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ŠPANOVÁ, A.TURKOVÁ, K. DRÁB, V. ŠPANO, M. BURDYCHOVÁ, R. RITTICH, B.
Original Title
Characterisation of potential probiotic Lactobacillus strains of human origin
Type
abstract
Language
English
Original Abstract
Molecular biological methods (genus-specific PCR, species-specific PCRs, RAPD with primer M13, and rep-PCR with primer (GTG)5) were applied for the identification and discrimination of 31 strains of the Lactobacillus genus isolated from faecal samples of 30 breastfed infants. All strains were tested for bile salts and low pH tolerance, growth in milk, production of lactic acids in milk supplemented with yeast extract, and used in biochemical tests including production of beta-glucuronidase. Consequently, the presence of genes coding for decarboxylases was investigated using PCR. Finally, four strains of probiotic species were selected for use in the dairy industry and for other tests concerning their probiotic character.
Keywords
Lactobacillus, identification, PCR, RAPD, rep-PCR, probiotics, in vitro tests
Authors
ŠPANOVÁ, A.;TURKOVÁ, K.; DRÁB, V.; ŠPANO, M.; BURDYCHOVÁ, R.; RITTICH, B.
Released
14. 9. 2010
Publisher
University of Bologna
Location
Rimini, Itálie
Pages count
1
BibTex
@misc{BUT73407, author="ŠPANOVÁ, A. and TURKOVÁ, K. and DRÁB, V. and ŠPANO, M. and BURDYCHOVÁ, R. and RITTICH, B.", title="Characterisation of potential probiotic Lactobacillus strains of human origin", booktitle="14th International Biotechnology Symposium Abstract Book", year="2010", pages="1", publisher="University of Bologna", address="Rimini, Itálie", note="abstract" }