Publication detail

Streptavidin-modified magnetic poly(2-hydroxyethyl methacrylate-co-g-lycidyl methacrylate) microspheres for selective isolation of bacterial DNA.

HORÁK, D., ŠPANOVÁ A., TVRDÍKOVÁ J.,RITTICH, B.

Original Title

Streptavidin-modified magnetic poly(2-hydroxyethyl methacrylate-co-g-lycidyl methacrylate) microspheres for selective isolation of bacterial DNA.

Type

journal article in Web of Science

Language

English

Original Abstract

The dispersion copolymerization of 2-hydroxyethyl methacrylate (HEMA) with glycidyl methacrylate (GMA) in a toluene/2-methylpropan-1-ol mixture in the presence of Fe3O4 nanoparticles coated with oleic acid, produced monodisperse magnetic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-GMA)) microspheres. Oxirane groups of the microspheres were ammonolyzed and then functionalized with streptavidin using cyanuric chloride. The final product contained 0.67 mg of streptavidin per gram of wet magnetic P(HEMA-GMA) microspheres. The microspheres were characterized by elemental analysis, scanning electron microscopy, IR, UV VIS and atomic absorption spectroscopy The streptavidin-modified magnetic P(HEMA-GMA) microspheres were used for immobilization of biotinylated DNA and subsequent selective isolation of target DNA from complex samples using DNA/DNA hybridisation.

Keywords

Glycidyl methacrylate; 2-hydroxyethyl methacrylate; Streptavidin; Biotin; Magnetic Microspheres; DNA

Authors

HORÁK, D., ŠPANOVÁ A., TVRDÍKOVÁ J.,RITTICH, B.

RIV year

2011

Released

30. 5. 2011

Publisher

PERGAMON-ELSEVIER SCIENCE

Location

Oxford

ISBN

0014-3057

Periodical

European Polymer Journal

Year of study

47

Number

5

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1090

Pages to

1096

Pages count

7

BibTex

@article{BUT73688,
  author="Bohuslav {Rittich} and Alena {Španová} and Jana {Tvrdíková}",
  title="Streptavidin-modified magnetic poly(2-hydroxyethyl methacrylate-co-g-lycidyl methacrylate) microspheres for selective isolation of bacterial DNA.",
  journal="European Polymer Journal",
  year="2011",
  volume="47",
  number="5",
  pages="1090--1096",
  issn="0014-3057"
}