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ŠURANSKÁ, H. VRÁNOVÁ, D. JIŘÍKOVÁ, I. PROCHÁZKOVÁ, L. VADKERTIOVÁ, R. OMELKOVÁ, J.
Original Title
PCR-RFLP; efficient method for non-Saccharomyces yeasts determination during production of integrated and organic wine
Type
abstract
Language
English
Original Abstract
The aim of this study was to identify yeasts isolated from red wine of Rulandske modre variety (Vitis vinifera) from integrated and ecological agriculture mode (production) from South Moravia. The yeast strains were isolated in regular intervals during spontaneous fermented must (without any SO2 addition). Together, we identified 124 strains (60 from ecological; 64 from integrated fermented must). As a rapid identification method, we used PCR-RFLP (Polymerase Chain Reaction and Restriction Fragment Length Polymorphism) and by application of specific primers (ITS1-ITS4) we amplified 5.8S-ITS segment of rDNA. PCR-RFLP of ITS regions using HaeIII, HinfI, TaqI, AluI and MseI (TruI) was used for identification and grouping of genetically similar strains. Restriction fragments were detected by agarose gel electrophoresis. All obtained electrophoreograms were processed by software BioNumerics 6.5 and UPGMA cluster analysis.
Keywords
wine, yeasts, PCR RFLP
Authors
ŠURANSKÁ, H.; VRÁNOVÁ, D.; JIŘÍKOVÁ, I.; PROCHÁZKOVÁ, L.; VADKERTIOVÁ, R.; OMELKOVÁ, J.
Released
28. 10. 2011
Publisher
Masarykova universita
Location
Brno
ISBN
978-80-210-5594-0
Book
XV. Setkání biochemiků a molekulárních biologů: Sborník příspěvků
Edition number
1
Pages from
95
Pages to
Pages count
BibTex
@misc{BUT74869, author="Hana {Šuranská} and Dana {Vránová} and Ivana {Jiříková} and Lenka {Procházková} and Renata {Vadkertiová} and Jiřina {Omelková}", title="PCR-RFLP; efficient method for non-Saccharomyces yeasts determination during production of integrated and organic wine", booktitle="XV. Setkání biochemiků a molekulárních biologů: Sborník příspěvků", year="2011", edition="1", pages="95--95", publisher="Masarykova universita", address="Brno", isbn="978-80-210-5594-0", note="abstract" }