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ZÍTKA, O. KŘÍŽKOVÁ, S. KREJČOVÁ, L. HYNEK, D. GUMULEC, J. MASAŘÍK, M. SOCHOR, J. ADAM, V. HUBÁLEK, J. TRNKOVÁ, L. KIZEK, R.
Original Title
Microfluidic tool based on the antibodymodified paramagnetic particles for detection of 8-hydroxy-2-deoxyguanosine in urine of prostate cancer patients
Type
journal article in Web of Science
Language
English
Original Abstract
A special modular electrochemical SFIA system which needs only 10 mL of sample including working buffer for one analysis was completely designed and successfully verified. The recoveries in different matrices and analyte concentration were estimated. Detection limit (3 S/N) was estimated as 5 pg/mL of 8-OHdG. This method promises to be very easily modified to microfluidic systems as lab on valve. The optimized method had sufficient selectivity and thus could be used for determination of 8-OHDG in human urine and therefore for estimation of oxidative DNA damage as a result of oxidation stress in prostate cancer patients.
Keywords
Electrochemistry, ELISA, Magnetic particles, Nanoseparation
Authors
ZÍTKA, O.; KŘÍŽKOVÁ, S.; KREJČOVÁ, L.; HYNEK, D.; GUMULEC, J.; MASAŘÍK, M.; SOCHOR, J.; ADAM, V.; HUBÁLEK, J.; TRNKOVÁ, L.; KIZEK, R.
RIV year
2011
Released
1. 9. 2011
Publisher
Wiley
ISBN
0173-0835
Periodical
Electrophoresis
Year of study
2011 (32)
Number
1
State
Federal Republic of Germany
Pages from
3207
Pages to
3220
Pages count
14
BibTex
@article{BUT75097, author="Ondřej {Zítka} and Soňa {Křížková} and Ludmila {Krejčová} and David {Hynek} and Jaromír {Gumulec} and Michal {Masařík} and Jiří {Sochor} and Vojtěch {Adam} and Jaromír {Hubálek} and Libuše {Trnková} and René {Kizek}", title="Microfluidic tool based on the antibodymodified paramagnetic particles for detection of 8-hydroxy-2-deoxyguanosine in urine of prostate cancer patients", journal="Electrophoresis", year="2011", volume="2011 (32)", number="1", pages="3207--3220", doi="10.1002/elps.201100430", issn="0173-0835" }