Publication detail

Effect of Nucleic Acid and Albumin on Luminescence Properties of Deposited TiO2 Quantum Dots

DRBOHLAVOVÁ, J. CHOMOUCKÁ, J. HRDÝ, R. PRÁŠEK, J. JANŮ, L. RYVOLOVÁ, M. ADAM, V. KIZEK, R. HALASOVÁ, T. HUBÁLEK, J.

Original Title

Effect of Nucleic Acid and Albumin on Luminescence Properties of Deposited TiO2 Quantum Dots

Type

journal article in Web of Science

Language

English

Original Abstract

Luminescent quantum dots (QDs) have gained a great attention as promising candidates in optical detection of various biomolecules such as DNA, proteins, etc. In this paper, we focused on fabrication of QDs in the deposited form, which still belongs to less clarified and used approach in biosensing compared to colloidal one. The deposited QDs from non-toxic titanium dioxide (TiO2) were prepared by template based technique, namely anodic oxidation of titanium film through nanoporous alumina template. This method allows to creation of QDs strongly fixed on silicon substrate without addition of any other fixation matrix (e.g. polymer). Highly ordered TiO2 QDs with broad luminescence peak in visible range were achieved. The QDs array was characterized by SEM confirming the QDs size being below 10 nm, which is necessary to reach the quantum size effect. Preliminary results showed that subsequent modification of QDs surface with chicken DNA and bovine serum albumin (BSA) resulted in partial or complete quenching of fluorescence depending on the biomolecules concentration.

Keywords

quantum dots; luminescence; biosensors; TiO2; template method; DNA; bovine serum albumin

Authors

DRBOHLAVOVÁ, J.; CHOMOUCKÁ, J.; HRDÝ, R.; PRÁŠEK, J.; JANŮ, L.; RYVOLOVÁ, M.; ADAM, V.; KIZEK, R.; HALASOVÁ, T.; HUBÁLEK, J.

RIV year

2012

Released

1. 2. 2012

Publisher

ESG

Location

Bělehrad, Srbsko

ISBN

1452-3981

Periodical

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

Year of study

7

Number

2

State

Republic of Serbia

Pages from

1424

Pages to

1432

Pages count

9

BibTex

@article{BUT75689,
  author="DRBOHLAVOVÁ, J. and CHOMOUCKÁ, J. and HRDÝ, R. and PRÁŠEK, J. and JANŮ, L. and RYVOLOVÁ, M. and ADAM, V. and KIZEK, R. and HALASOVÁ, T. and HUBÁLEK, J.",
  title="Effect of Nucleic Acid and Albumin on Luminescence Properties of Deposited TiO2 Quantum Dots",
  journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE",
  year="2012",
  volume="7",
  number="2",
  pages="1424--1432",
  issn="1452-3981"
}