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KLECKEROVÁ, A. SOBROVÁ, P. KRYŠTOFOVÁ, O. SOCHOR, J. ZÍTKA, O. BABULA, P. ADAM, V. DOČEKALOVÁ, H. KIZEK, R.
Original Title
Cadmium(II) and zinc(II) ions effects on maize plants revealed by spectroscopy and electrochemistry
Type
journal article in Web of Science
Language
English
Original Abstract
Toxicity of cadmium has been described in numerous papers and includes especially ability to generate reactive oxygen species (ROS), which are responsible for damaging of biomolecules and loss of their biological functions, however, mutual effect of zinc(II) and cadmium(II) ions is still unclear. Growth parameters (root/shoot length, fresh and dry weight), and stress markers and enzymes (oxidized/reduced glutathione, phytochelatins, total content of thiols, activities of glutathione-S-transferase (GST), alanine transaminase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP)) were monitored during the treatment. In addition, total content of zinc(II) and cadmium(II) ions was determined in plant tissues electrochemically using differential pulse anodic stripping voltammetry. Moreover, content of reduced (GSH) and oxidized (GSSG) glutathione, and phytochelatin2 and phytochelatin5 were quantified using high performance liquid chromatography with electrochemical detection (HPLC-ED). Firstly, we studied the influence of metal ions on the growth parameters, i.e. fresh/dry weight and root/shoot length. Application of both metals led to the reduction of root as well as shoot biomass compared to control untreated plants. These adverse effects were found also in length of shoot and roots as well as dry weight. Content of both cadmium and zinc was determined in roots and shoots of experimental plants using Differential Pulse Anodic Stripping Voltammetry (DPASV). Amount of thiol compounds as well as GST activity was two times higher in roots compared to shoots. Aiming our attention on content of particular thiols, significant increasing of total content of GSH was observed at both 2nd and 10th day of the treatment. The most significant changes were evident in the case of the highest joint concentrations of zinc(II) and cadmium(II) ions - 100 uM Zn2+ + 100 uM Cd2+. Treatment with both cadmium(II) and/or zinc(II) ions led to the increase of PC2 and PC5 in experimental plants. Higher amount of phytochelatins in shoots compared to roots indicates their role in chelatation and transport of both heavy metals to aerial parts.
Keywords
stress markers, glutathione-S-transferase, alanine transaminase, aspartate transaminase, alkaline phosphatase, Differential Pulse Anodic Stripping Voltammetry
Authors
KLECKEROVÁ, A.; SOBROVÁ, P.; KRYŠTOFOVÁ, O.; SOCHOR, J.; ZÍTKA, O.; BABULA, P.; ADAM, V.; DOČEKALOVÁ, H.; KIZEK, R.
RIV year
2011
Released
1. 12. 2011
ISBN
1452-3981
Periodical
INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE
Year of study
2011 (6)
Number
12
State
Republic of Serbia
Pages from
6011
Pages to
6031
Pages count
21
BibTex
@article{BUT75729, author="Andrea {Ridošková} and Pavlína {Adam} and Olga {Kryštofová} and Jiří {Sochor} and Ondřej {Zítka} and Petr {Babula} and Vojtěch {Adam} and Hana {Dočekalová} and René {Kizek}", title="Cadmium(II) and zinc(II) ions effects on maize plants revealed by spectroscopy and electrochemistry", journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE", year="2011", volume="2011 (6)", number="12", pages="6011--6031", issn="1452-3981" }