Publication result detail

Impedimetric Aptasensor for Thrombin Recognition Based on CD Support

CASTILLO, G.; TRNKOVÁ, L.; HRDÝ, R.; HIANIK, T.

Original Title

Impedimetric Aptasensor for Thrombin Recognition Based on CD Support

English Title

Impedimetric Aptasensor for Thrombin Recognition Based on CD Support

Type

WoS Article

Original Abstract

We report an aptamer biosensing array for thrombin detection by measuring the electrochemical impedance upon aptamer-protein formation at the surface of CD-trodes (GCDTs) in the presence of the redox couple [Fe(CN)6]3/4. GCDTs are fabricated from recordable compact discs that contain a fine gold layer. The biosensor is constructed by self-assembling of a thiol-modified thrombin binding aptamer (TBA) onto a GCDT surface. The sensor reveals good ligand specificity, recognition in a wide range of thrombin concentrations from 20 nM to 1 mM with a limit of detection of 5 nM

English abstract

We report an aptamer biosensing array for thrombin detection by measuring the electrochemical impedance upon aptamer-protein formation at the surface of CD-trodes (GCDTs) in the presence of the redox couple [Fe(CN)6]3/4. GCDTs are fabricated from recordable compact discs that contain a fine gold layer. The biosensor is constructed by self-assembling of a thiol-modified thrombin binding aptamer (TBA) onto a GCDT surface. The sensor reveals good ligand specificity, recognition in a wide range of thrombin concentrations from 20 nM to 1 mM with a limit of detection of 5 nM

Keywords

DNA aptamers, Biosensors, Human thrombin, Electrochemical impedance spectroscopy

Key words in English

DNA aptamers, Biosensors, Human thrombin, Electrochemical impedance spectroscopy

Authors

CASTILLO, G.; TRNKOVÁ, L.; HRDÝ, R.; HIANIK, T.

RIV year

2013

Released

01.05.2012

ISBN

1040-0397

Periodical

ELECTROANALYSIS

Volume

24

Number

5

State

Federal Republic of Germany

Pages from

1079

Pages to

1087

Pages count

9

BibTex

@article{BUT92982,
  author="Gabriela {Castillo} and Libuše {Trnková} and Radim {Hrdý} and Tibor {Hianik}",
  title="Impedimetric Aptasensor for Thrombin Recognition Based on CD Support",
  journal="ELECTROANALYSIS",
  year="2012",
  volume="24",
  number="5",
  pages="1079--1087",
  doi="10.1002/elan.201200041",
  issn="1040-0397"
}