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ZÍTKA, O. BABULA, P. SOCHOR, J. KUMMEROVÁ, M. KRYŠTOFOVÁ, O. ADAM, V. HAVEL, L. BEKLOVÁ, M. HUBÁLEK, J. KIZEK, R.
Original Title
Determination of Eight Polycyclic Aromatic Hydrocarbons and in Pea Plants (Pisum sativum L.) Extracts by High Performance Liquid Chromatography with Electrochemical Detection
Type
journal article in Web of Science
Language
English
Original Abstract
In the field of plant physiology, knowledge about polycyclic aromatic hydrocarbons (PAHs), mechanisms of actions are still missing and are limited despite the fact that induction of oxidative stress by several PAHs has been demonstrated. Primarily, high performance liquid chromatography coupled with UV detector and high performance liquid chromatography coupled with electrochemical detector were optimized for detection and quantification of the following PAHs as fluoranthene, pyrene, benzo[a]pyrene, naphthalene, benzo[a]antracene, methylanthracene, triphenylene and coronene. Optimised HPLC method enabled determination of fluoranthene transport into different plant organs - roots, stems and leaves. Hydroponically cultivated pea plants also proved as suitable model for monitoring of effect of fluoranthene on uptake and transport of physiologically crucial elements as calcium, copper, iron and zinc. Significant differences between control and treated plants were determined, however, role of these elements in responses to fluoranthene treatment should be further investigated.
Keywords
Polycyclic Aromatic Hydrocarbons; High Performance Liquid Chromatography with UV and Electrochemical Detection; Fluoranthene; Pea Plants; Pisum sativum
Authors
ZÍTKA, O.; BABULA, P.; SOCHOR, J.; KUMMEROVÁ, M.; KRYŠTOFOVÁ, O.; ADAM, V.; HAVEL, L.; BEKLOVÁ, M.; HUBÁLEK, J.; KIZEK, R.
RIV year
2012
Released
1. 2. 2012
ISBN
1452-3981
Periodical
INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE
Year of study
7
Number
2
State
Republic of Serbia
Pages from
908
Pages to
927
Pages count
20
BibTex
@article{BUT92984, author="Ondřej {Zítka} and Petr {Babula} and Jiří {Sochor} and Marie {Kummerová} and Olga {Kryštofová} and Vojtěch {Adam} and Ladislav {Havel} and Miroslava {Beklová} and Jaromír {Hubálek} and René {Kizek}", title="Determination of Eight Polycyclic Aromatic Hydrocarbons and in Pea Plants (Pisum sativum L.) Extracts by High Performance Liquid Chromatography with Electrochemical Detection", journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE", year="2012", volume="7", number="2", pages="908--927", issn="1452-3981" }