Publication detail

Automated assay of the potency of natural antioxidants using pipetting robot and spectrophotometry

POHANKA, M. SOCHOR, J. RUTTKAY-NEDECKÝ, B. CERNEI, N. ADAM, V. HUBÁLEK, J. STIBOROVÁ, M. ECKSCHLAGER, T. KIZEK, R.

Original Title

Automated assay of the potency of natural antioxidants using pipetting robot and spectrophotometry

Type

journal article in Web of Science

Language

English

Original Abstract

In the food industry, in the process of creating new agricultural plant products, and in the testing of anti-cancer drugs there is often a need to assay multiple samples of low molecular weight antioxidants, plant samples and foods rich in antioxidants, with minimal additional costs and low degrees of uncertainty. With these demands in mind, we decided to study the fully automated assay of antioxidants using not only automated sample measurements but also automated processing of samples and application of reagents. The automated pipetting system epMotion 5075 and the automated spectrophotometer BS 400 were chosen for the assay purposes.

Keywords

DPPH; FRAP; ABTS; DMPD; free radicals; antioxidant

Authors

POHANKA, M.; SOCHOR, J.; RUTTKAY-NEDECKÝ, B.; CERNEI, N.; ADAM, V.; HUBÁLEK, J.; STIBOROVÁ, M.; ECKSCHLAGER, T.; KIZEK, R.

RIV year

2012

Released

17. 5. 2012

Publisher

University of South Bohemia

ISBN

1214-0287

Periodical

Journal of Applied Biomedecine

Year of study

10

Number

3

State

Czech Republic

Pages from

155

Pages to

167

Pages count

13

URL

https://jab.zsf.jcu.cz/artkey/jab-201203-0004_automated-assay-of-the-potency-of-natural-antioxidants-using-pipetting-robot-and-spectrophotometry.php

Full text in the Digital Library

http://hdl.handle.net/11012/200907

BibTex

@article{BUT92998,
  author="Miroslav {Pohanka} and Jiří {Sochor} and Branislav {Ruttkay-Nedecký} and Natalia Vladimirovna {Cernei} and Vojtěch {Adam} and Jaromír {Hubálek} and Marie {Stiborová} and Tomáš {Eckschlager} and René {Kizek}",
  title="Automated assay of the potency of natural antioxidants using pipetting robot and spectrophotometry",
  journal="Journal of Applied Biomedecine",
  year="2012",
  volume="10",
  number="3",
  pages="155--167",
  doi="10.2478/v10136-012-0006-y",
  issn="1214-0287",
  url="https://jab.zsf.jcu.cz/artkey/jab-201203-0004_automated-assay-of-the-potency-of-natural-antioxidants-using-pipetting-robot-and-spectrophotometry.php"
}