Publication detail

Identification and determination of relatedness of lactobacilli using different DNA amplification methods

TURKOVÁ, K. RITTICH, B. ŠPANOVÁ, A.

Original Title

Identification and determination of relatedness of lactobacilli using different DNA amplification methods

Type

journal article - other

Language

English

Original Abstract

Several DNA amplification-based methods were used for identification and evaluation of the relation between lactobacilli isolated from breastfed full-term infant faeces, dairy products and silage. Twenty-seven strains isolated friom infant faeces were identified as Lactobacillus rhamnosus, Lactobacillus gasseri, Lactobacillus paracasei, Lactobacillus fermentum, Lactobacillus salivarius, Lactobacillus plantarum, and Lactobacillus helveticus using ten species-specific PCRs, multiplex PCR, and sequencing of 16SrDNA. Four strains were not identified. A repetitive extragenic palindromic PCR (rep-PCR) and randomly amplified polymorphic DNA PCR (RAPD-PCR) were used for confirmation of species identification. Fingerprints were used for evaluation of the relatedness of lactobacilli. Differences between strains from infant faeces, dairy products, and silage were not detected.

Keywords

Lactobacillus, breastfed infants, dairy products, identification, PCR, fingerprinting

Authors

TURKOVÁ, K.; RITTICH, B.; ŠPANOVÁ, A.

RIV year

2012

Released

1. 9. 2012

Publisher

Springer

ISBN

0366-6352

Periodical

Chemical Papers

Year of study

66

Number

9

State

Slovak Republic

Pages from

842

Pages to

851

Pages count

10

BibTex

@article{BUT93027,
  author="Kristýna {Turková} and Bohuslav {Rittich} and Alena {Španová}",
  title="Identification and determination of relatedness of lactobacilli using different DNA amplification methods",
  journal="Chemical Papers",
  year="2012",
  volume="66",
  number="9",
  pages="842--851",
  issn="0366-6352"
}