Publication detail

Effect of season, needle age and elevated CO2 concentration on photosynthesis and Rubisco acclimation in Picea abies

URBAN, O. HRSTKA, M. ZITOVÁ, M. HOLIŠOVÁ, P. ŠPRTOVÁ, M. KLEM, K. CALFAPIETRA, C. DE ANGELIS, P. MAREK, M.

Original Title

Effect of season, needle age and elevated CO2 concentration on photosynthesis and Rubisco acclimation in Picea abies

Type

journal article - other

Language

English

Original Abstract

We comprehensively studied Rubisco adjustment to EC in coniferous Picea abies using an electrophoretic (protein content), spectrophotometric (initial (RAinitial) and total (RAtotal) in vitro Rubisco activities), and gas-exchange (maximum carboxylation activity in vivo (VCmax)) techniques. With respect to differing carbon sink strength and nitrogen remobilization, we hypothesized greater acclimation of photosynthesis in one-year-old as compared to current-year needles and at the end than at the beginning of the vegetation season. EC treatment led to a decrease in VCmax values in current-year needles, but the ribulose1,5-bis- phosphate (RuBP)-limited rate of photosynthesis (Jmax) remained unaffected. Indeed, both VCmax and Jmax were reduced by the EC treatment in one-year-old needles. The extent of photosynthetic acclimation in EC plants did not increase, however, during the vegetation season. EC decreased the activation state of Rubisco (RAinitial/RAtotal) by 16% and 5% in current-year and one-year-old needles, respectively (averaged over the growing season). While during spring (short-term effect) EC treatment did not influence the Rubisco content per unit leaf area and decreased its specific activity (activity per unit Rubisco mass) in both needle age classes studied, exposure to EC during the entire vegetation season tended to reduce the Rubisco content while increasing its specific activity. Irrespective of CO2 treatment and needle age, a hyperbolic-decay relationship was observed between Rubisco-specific activity and its content.

Keywords

activation state, electron transport rate, Norway spruce, photosynthetic acclimation, Rubisco carboxylation, Rubisco specific activity

Authors

URBAN, O.; HRSTKA, M.; ZITOVÁ, M.; HOLIŠOVÁ, P.; ŠPRTOVÁ, M.; KLEM, K.; CALFAPIETRA, C.; DE ANGELIS, P.; MAREK, M.

RIV year

2012

Released

5. 7. 2012

Publisher

Elsevier

ISBN

0981-9428

Periodical

PLANT PHYSIOLOGY AND BIOCHEMISTRY

Year of study

58

Number

--

State

French Republic

Pages from

135

Pages to

141

Pages count

7

BibTex

@article{BUT93414,
  author="Otmar {Urban} and Miroslav {Hrstka} and Martina {Zitová} and Petra {Holišová} and Mirka {Šprtová} and Karel {Klem} and Carlo {Calfapietra} and Paolo {De Angelis} and Michal V. {Marek}",
  title="Effect of season, needle age and elevated CO2 concentration on photosynthesis and Rubisco acclimation in Picea abies",
  journal="PLANT PHYSIOLOGY AND BIOCHEMISTRY",
  year="2012",
  volume="58",
  number="--",
  pages="135--141",
  issn="0981-9428"
}