Publication detail

The influence of mechanical stimulation on osteoclast localization in the mouse maxilla: bone histomorphometry and finite element analysis

FUJIKI, K. AOKI, K. MARCIÁN, P. BORÁK, L. HUDIEB, M. OHYA, K. IGARASHI, Y. WAKABAYASHI, N.

Original Title

The influence of mechanical stimulation on osteoclast localization in the mouse maxilla: bone histomorphometry and finite element analysis

Type

journal article - other

Language

English

Original Abstract

The mechanism of traumatic bone resorption in the denture-bearing bone has not yet been established with regard to the osteoclastic activity in relation to the mechanical stimulus. The purpose of this studywas to clarify whether osteoclast appearance in maxilla depends on the strain intensity, using themurine loading model. The maxillary palate of thirteen-week-old male C57BL/6 mice was subjected to continuous pressure of 2 kPa (low stimulation, n = 4) or 7 kPa (high stimulation, n = 4) for 30min/day for 7 consecutive days, and the mice were sacrificed after the last loading. The control group underwent the same protocol without load (n = 4). An animal-specific finite element model was constructed based on morphology and characteristics obtained from the micro-CT data and used to calculate the strain intensity of the bone. The bone histomorphometric technique revealed significant reduction of cortical bone volume and significant increase of bone resorption parameters such as osteoclast number in the bone tissue under the loading contact in comparison to the control (p < 0.05). The osteoclasts were observed in the subsurface region adjacent to the loading contact and the peripheral region of the marrow space in the intracortical region of the cortical bone in the mouse maxilla in both stimulation groups. An average of more than 90% of the osteoclasts was observed in the areas with strain intensity higher than 85.0 micro strain for the high stimulation group. The result suggests that the osteoclastic resorption is location-dependent and is also sensitive to the local strain intensity.

Keywords

Bone; Osteoclast; Mechanical stimulation; Finite element analysis; Strain

Authors

FUJIKI, K.; AOKI, K.; MARCIÁN, P.; BORÁK, L.; HUDIEB, M.; OHYA, K.; IGARASHI, Y.; WAKABAYASHI, N.

RIV year

2013

Released

1. 4. 2013

Publisher

Springer

Location

Germany

ISBN

1617-7959

Periodical

Biomechanics and Modeling in Mechanobiology

Year of study

12

Number

2

State

Federal Republic of Germany

Pages from

325

Pages to

333

Pages count

9

URL

http://link.springer.com/article/10.1007/s10237-012-0401-z

BibTex

@article{BUT94207,
  author="Kengo {Fujiki} and Kazuhiro {Aoki} and Petr {Marcián} and Libor {Borák} and Malik {Hudieb} and Keiichi {Ohya} and Yoshimasa {IGARASHI} and Noriyuki {WAKABAYASHI}",
  title="The influence of mechanical stimulation on osteoclast localization in the mouse maxilla: bone histomorphometry and finite element analysis",
  journal="Biomechanics and Modeling in Mechanobiology",
  year="2013",
  volume="12",
  number="2",
  pages="325--333",
  issn="1617-7959",
  url="http://link.springer.com/article/10.1007/s10237-012-0401-z"
}