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PEKAROVÁ, M. KUCHTA, R. KADLEC, J.
Original Title
The unique role of dietary l-arginine in the acceleration of peritoneal macrophage sensitivity to bacterial endotoxin
Type
journal article in Web of Science
Language
English
Original Abstract
It is known that cells and organisms can indirectly sense changes in l-arginine availability via changes in the activity of various metabolic pathways. However, the mechanism(s) by which genes can be directly regulated by l-arginine in mammalian cells have not yet been elucidated. We investigated the effect of l-arginine in the in vivo model of peritoneal inflammation in mice and in vitro in RAW 264.7 macrophages. A detailed analysis of basic physiological functions and selected intracellular signaling cascades revealed that l-arginine is crucial for the acceleration of macrophage activation by bacterial lipopolysaccharide. l-arginine increased the production of reactive oxygen species, nitric oxide, release of Ca2+, as well as inducible nitric oxide synthase expression. Interestingly, the effect of l-arginine on macrophage activation was dependent on the phosphorylation of mitogen-activated protein kinases and activity of phospholipase C. In RAW 264.7 cells, l-arginine was shown to modulate the response of macrophages toward lipopolysaccharide via the activation of G-protein-coupled receptors. According to our data, we concluded that l-arginine availability plays a key role in the initiation of intracellular signaling pathways that trigger the lipopolysaccharide-induced inflammatory responses in murine macrophages. Although macrophages are partially stimulated in the absence of extracellular l-arginine, the presence of this amino acid significantly accelerates the sensitivity of macrophages to bacterial endotoxin.
Keywords
Macrophages; l-arginine; Lipopolysaccharide; Mitogen-activated protein kinases; Phospholipase C; G-protein-coupled receptor
Authors
PEKAROVÁ, M.; KUCHTA, R.; KADLEC, J.
RIV year
2013
Released
27. 11. 2012
Publisher
PublisherSpringer-Verlag
ISBN
0257-277X
Periodical
IMMUNOLOGIC RESEARCH
Year of study
2012
Number
11
State
United States of America
Pages from
2443
Pages to
2457
Pages count
12
BibTex
@article{BUT95688, author="Michaela {Pekarová} and Radek {Kuchta} and Jaroslav {Kadlec} and Zdeňka {Kuchtová}", title="The unique role of dietary l-arginine in the acceleration of peritoneal macrophage sensitivity to bacterial endotoxin", journal="IMMUNOLOGIC RESEARCH", year="2012", volume="2012", number="11", pages="2443--2457", issn="0257-277X" }