Publication detail

Effect of organic and inorganic form of selenium on antioxidant status of breeding boars ejaculate revealed by electrochemistry

HORKY,P.,JANCIKOVA,P., SOCHOR, J.,HYNEK, D. CHAVIS, G.J., C. RUTTKAY-NEDECKÝ, B. CERNEI, N. ZÍTKA, O. ZEMAN, L. ADAM, V. KIZEK, R.

Original Title

Effect of organic and inorganic form of selenium on antioxidant status of breeding boars ejaculate revealed by electrochemistry

Type

journal article in Web of Science

Language

English

Original Abstract

The main aim of the presented study was to determine the effects of selenium on antioxidant status of Duroc boars ejaculates. Twenty-eight Duroc boars were divided to four experimental groups, seven boars per each group. Each group was supplemented with both the organic form (0.3 and 0.6 mg selenium per kg) and inorganic form (0.3 and 0.6 mg selenium per kg) of selenium in the feed mixture. The duration of the experiment was 18 weeks. All the boars were carefully collected at strictly defined four time intervals. The activity of glutathione peroxidase, glutathione reductase, antioxidant capacity, ratio between reduced and oxidized glutathione, metallothionein content and concentration of zinc and selenium were determined. The dietary supplementation of the organic form of selenium at a dose of 0.6 mg/kg of feed mixture increased significantly (by 48 %, P < 0.01) activity of the glutathione peroxidase in the ejaculate. Concentration of selenium in ejaculate of this experimental group was increased by 82 % (P < 0.05). Dietary supplementation of organic form of selenium at a dose of 0.6 mg/kg of feed mixture led to an increase of free radicals by 62 % (P < 0.01), ABTS for 45 % (P < 0.05), and GSH/GSSH for 55 % (P < 0.05). When evaluating the content of zinc and metallothionein, no significant differences between experimental groups of animals were found. Experimental group of boars supplemented by the selenium in the concentration of 0.3 mg/kg in the feed mixture in the inorganic form had significantly reduced the total number of sperms produced per one taking (P < 0.001). The results of the present study indicate that the dietary supplementation of feed mixture by the selenium in the concentration of 0.6 mg Se/kg significantly improves the antioxidant potential of the breeding boars ejaculate. A lower oxidative stress and higher fertility can be therefore assumed.

Keywords

selenomethionine; sodium selenite; antioxidant status; zinc; metallothionei boars; differential pulse voltammetry; high performance liquid chromatography with electrochemical detection

Authors

HORKY,P.,JANCIKOVA,P., SOCHOR, J.,HYNEK, D.; CHAVIS, G.J., C.; RUTTKAY-NEDECKÝ, B.; CERNEI, N.; ZÍTKA, O.; ZEMAN, L.; ADAM, V.; KIZEK, R.

RIV year

2012

Released

1. 10. 2012

Publisher

ELECTROCHEMICAL SCIENCE GROUP, A SPOMENICE 7/12 , 19210 BOR, BELGRADE, VJ 12, SERBIA

ISBN

1452-3981

Periodical

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

Year of study

7

Number

10

State

Republic of Serbia

Pages from

9643

Pages to

9657

Pages count

14

BibTex

@article{BUT98588,
  author="David {Hynek} and Jiří {Sochor} and Branislav {Ruttkay-Nedecký} and Natalia Vladimirovna {Cernei} and Ondřej {Zítka} and Ladislav {Zeman} and Vojtěch {Adam} and René {Kizek}",
  title="Effect of organic and inorganic form of selenium on antioxidant status of breeding boars ejaculate revealed by electrochemistry",
  journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE",
  year="2012",
  volume="7",
  number="10",
  pages="9643--9657",
  issn="1452-3981"
}