Publication detail

Carbofuran assay using Gelatin based Biosensor with Acetylcholinesterase as a Recognition Element

POHANKA, M. FUSEK, J. ADAM, V. KIZEK, R.

Original Title

Carbofuran assay using Gelatin based Biosensor with Acetylcholinesterase as a Recognition Element

Type

journal article in Web of Science

Language

English

Original Abstract

Carbofuran is a neurotoxic pesticide acting as an inhibitor of enzyme acetylcholinesterase (AChE). The fact that carbofuran is able to inhibit AChE can be used for its assay using an appropriate sensor. AChE was immobilized into gelatin membrane. The immobilization procedure was optimized considering temperature of drying and concentration of gelatin in mixture. Long term stability and interference of organic solvent was done as well. In the final experiment, carbofuran was assayed using the prepared biosensor. Limit of detection 5.01 10-10 mol/l (0.111 ng/ml) was achieved.

Keywords

biosensor; acetylcholinesterase; pesticide; inhibitor; screen printed electrode; voltammetry; amperometry

Authors

POHANKA, M.; FUSEK, J.; ADAM, V.; KIZEK, R.

RIV year

2013

Released

1. 1. 2013

ISBN

1452-3981

Periodical

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

Year of study

8

Number

1

State

Republic of Serbia

Pages from

71

Pages to

79

Pages count

9

BibTex

@article{BUT98896,
  author="Miroslav {Pohanka} and Josef {Fusek} and Vojtěch {Adam} and René {Kizek}",
  title="Carbofuran assay using Gelatin based Biosensor with Acetylcholinesterase as a Recognition Element",
  journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE",
  year="2013",
  volume="8",
  number="1",
  pages="71--79",
  issn="1452-3981"
}