Publication detail

Local Treatment of Hand-Foot Syndrome with Uridine/Thymidine:In Vitro Appraisal on a Human Keratinocyte Cell Line HaCaT

HARTINGER, J. VESELÝ, P. MATOUŠKOVÁ, E. ARGALACSOVÁ, S. PETRUŽELKA, L. NETÍKOVÁ, I.

Original Title

Local Treatment of Hand-Foot Syndrome with Uridine/Thymidine:In Vitro Appraisal on a Human Keratinocyte Cell Line HaCaT

Type

journal article in Web of Science

Language

English

Original Abstract

5-fluorouracil (5-FU) is one of the most commonly used antineoplastic drugs in the anticancer therapy. The hand-foot (HF) syndrome (palmar-plantar erythrodysesthesia) is an adverse effect frequently related to long-term i.v. administration of 5-FU or its orally applicable prodrug capecitabine. Its severity can even lead to interruption of the otherwise effective anticancer therapy. Tentative practice in some clinics has shown that topical application of 10% uridine ointment is beneficial for calming down the HF syndrome. This study is focused on verifying the alleged protective activity of uridine in the in vitro model of cultured human keratinocyte cell line HaCaT. We also tested the protective effects of thymidine alone or uridine-thymidine combination. The cellular viability time progression was measured in order to evaluate the effect of protective agents by three different types of cytopathogenicity tests - NTCA test (non-destructive test of cellular activity), modified MTT test and RTCA (real-time cell analyser, Roche). All three methods proved the ability of uridine and uridine-thymidine combination to protect keratinocytes against 5-FU damage in vitro. While thymidine alone did not show any remarkable effect, the thymidine-uridine combination demonstrated enhanced protective activity compared to uridine alone. Our findings provided the supporting rationale for using uridine or uridine-thymidine ointments in the HF syndrome local therapy.

Keywords

5-fluorouracil Hand-Foot syndrom uridine thymidine

Authors

HARTINGER, J.; VESELÝ, P.; MATOUŠKOVÁ, E.; ARGALACSOVÁ, S.; PETRUŽELKA, L.; NETÍKOVÁ, I.

RIV year

2012

Released

31. 7. 2012

Publisher

Hindawi

ISBN

1537-744X

Periodical

The Scientific World Journal

Year of study

2012

Number

7

State

United States of America

Pages from

1

Pages to

6

Pages count

6

URL

https://www.hindawi.com/journals/tswj/2012/421325/

Full text in the Digital Library

http://hdl.handle.net/11012/84150

BibTex

@article{BUT99043,
  author="Jan {Hartinger} and Pavel {Veselý} and Eva {Matoušková} and Soňa {Argalacsová} and Luboš {Petruželka} and Irena {Štenglová Netíková}",
  title="Local Treatment of Hand-Foot Syndrome with Uridine/Thymidine:In Vitro Appraisal on a Human Keratinocyte Cell Line HaCaT",
  journal="The Scientific World Journal",
  year="2012",
  volume="2012",
  number="7",
  pages="1--6",
  doi="10.1100/2012/421325",
  issn="1537-744X",
  url="https://www.hindawi.com/journals/tswj/2012/421325/"
}