Publication detail

Conjugation Bovine Serum Albumine with CdTe Quantum Dots

CHOMOUCKÁ, J. DRBOHLAVOVÁ, J. MAJZLÍKOVÁ, P. PRÁŠEK, J. PEKÁREK, J. HRDÝ, R. HUBÁLEK, J.

Original Title

Conjugation Bovine Serum Albumine with CdTe Quantum Dots

Type

conference paper

Language

English

Original Abstract

There is a growing interest in using semiconductor quantum dots (QDs) as optical labels for biosensing events. QDs have been linked with bio-recognition molecules such as proteins, peptides and nucleic acids, and have been successfully used in biological and medical fields such as immunoassay, DNA hybridization, cell imaging and potential photodynamic therapy. In this paper, the interaction between bovine serum albumin (BSA) and water soluble CdTe QDs modified with different ligands (3-mercaptoacetic acid, thioglycolic acid and glutathione) was studied using the fluorescence spectroscopy. It was found that the presence of QDs led to a strong quenching effect of BSA, which could be explained by a covalent interaction between the protein and the quencher, demonstrating the formation of QDs-BSA bioconjugates.

Keywords

Quantum dots, conjugation, bovine serum albumin

Authors

CHOMOUCKÁ, J.; DRBOHLAVOVÁ, J.; MAJZLÍKOVÁ, P.; PRÁŠEK, J.; PEKÁREK, J.; HRDÝ, R.; HUBÁLEK, J.

Released

16. 10. 2013

Publisher

Tanger, Ltd.

Location

Ostrava

ISBN

978-80-87294-47-5

Book

Conference Proceedings 5th International Conference NANOCON 2013

Edition

1

Edition number

1

ISBN

NEUVEDENO

Pages from

566

Pages to

571

Pages count

6

BibTex

@inproceedings{BUT102875,
  author="Jana {Pekárková} and Jana {Drbohlavová} and Petra {Majzlíková} and Jan {Prášek} and Jan {Pekárek} and Radim {Hrdý} and Jaromír {Hubálek}",
  title="Conjugation Bovine Serum Albumine with CdTe Quantum Dots",
  booktitle="Conference Proceedings 5th International Conference NANOCON 2013",
  year="2013",
  series="1",
  number="1",
  pages="566--571",
  publisher="Tanger, Ltd.",
  address="Ostrava",
  isbn="978-80-87294-47-5"
}