Publication detail

Electrochemical Microarray for Identification Pathogens: A Review

MERLOS RODRIGO, M. ZÍTKA, O. KREJČOVÁ, L. HYNEK, D. MASAŘÍK, M. KYNICKÝ, J. HEGER, Z. ADAM, V. KIZEK, R.

Original Title

Electrochemical Microarray for Identification Pathogens: A Review

Type

journal article in Web of Science

Language

English

Original Abstract

Numerous analytical methods are used to detect pathogens agents including methods based on the direct isolation followed by real time-polymerase chain reaction, or immunology tests. There have not been published numerous papers on the using of electrochemical microarrays for detection of viruses and/or bacteria but there is a great potential. Electrochemical detection has been developed and assay performances studied for the CombiMatrix oligonucleotide microarray platform. CombiMatrix core technology is based on a specially modified semiconductor adapted for biological applications, which contains arrays of platinum microelectrodes. The ElectraSenseTM principle is the detection of redox active chemistries proximal to specific electrodes. The target molecules are labelled with a redox enzyme via biotin–streptavidin (or avidin) interaction. Enzymatic oxidation of an electron donor substrate then occurs. The detection current is generated due to electro-reduction of the enzymatic reaction product. Thus, the ElectraSense platform has been used to develop nucleic acid assays for highly accurate genotyping of a variety of pathogens including bio-threat agents. In this review we summarize recent studies on the detection and identification of pathogens by electrochemical microarrays technology and discuss the advantages and disadvantages

Keywords

Electrochemical detection; Microarray platform; Viruses, Influenza virus; Human papillomavirus.

Authors

MERLOS RODRIGO, M.; ZÍTKA, O.; KREJČOVÁ, L.; HYNEK, D.; MASAŘÍK, M.; KYNICKÝ, J.; HEGER, Z.; ADAM, V.; KIZEK, R.

RIV year

2014

Released

1. 7. 2014

ISBN

1452-3981

Periodical

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

Year of study

9

Number

7

State

Republic of Serbia

Pages from

3431

Pages to

3439

Pages count

9

BibTex

@article{BUT109363,
  author="Miguel Ángel {Merlos Rodrigo} and Ondřej {Zítka} and Ludmila {Krejčová} and David {Hynek} and Michal {Masařík} and Jindřich {Kynický} and Zbyněk {Heger} and Vojtěch {Adam} and René {Kizek}",
  title="Electrochemical Microarray for Identification Pathogens: A Review",
  journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE",
  year="2014",
  volume="9",
  number="7",
  pages="3431--3439",
  issn="1452-3981"
}