Publication detail

Effect of CTAB and CTAB in the presence of hyaluronan on selected human cell types

KALBÁČOVÁ, M. VERDÁNOVÁ, M. MRAVEC, F. HALASOVÁ, T. PEKAŘ, M.

Original Title

Effect of CTAB and CTAB in the presence of hyaluronan on selected human cell types

Type

journal article in Web of Science

Language

English

Original Abstract

The effect of different concentrations of CTAB (in the range of 0.2 mM – 2 mM, i.e. including its critical micelle concentration) on viability of selected human cells (osteoblasts and keratinocytes) was studied by a variety of methods (immunocytochemical and biochemical), testing the cell viability and the metabolism, to get a complex overview. All of the used methods confirmed the cytotoxic effect of CTAB, which could, however, be suppressed by the presence of hyaluronan (molecular weight 806 kDa, in the concentration of 1 g/l) in the case of the lowest CTAB concentration used (0.2 mM) when the fetal bovine serum was also present in the cultivation medium. Thus it could be concluded that hyaluronan can be used as successful protector of specific cell types against cytotoxic CTAB at low concentrations.

Keywords

Cetyltrimethylammonium bromide; Cytoprotection; Cytotoxicity; Hyaluronan; Keratinocyte; Osteoblast

Authors

KALBÁČOVÁ, M.; VERDÁNOVÁ, M.; MRAVEC, F.; HALASOVÁ, T.; PEKAŘ, M.

RIV year

2014

Released

8. 9. 2014

ISBN

0927-7757

Periodical

Colloids and Surfaces A: Physicochemical and Engineering Aspects

Year of study

460

Number

1

State

Kingdom of the Netherlands

Pages from

204

Pages to

208

Pages count

5

BibTex

@article{BUT109678,
  author="KALBÁČOVÁ, M. and VERDÁNOVÁ, M. and MRAVEC, F. and HALASOVÁ, T. and PEKAŘ, M.",
  title="Effect of CTAB and CTAB in the presence of hyaluronan on selected human cell types",
  journal="Colloids and Surfaces A: Physicochemical and Engineering Aspects",
  year="2014",
  volume="460",
  number="1",
  pages="204--208",
  issn="0927-7757"
}