Publication detail

Preparation of CuO/ZnO nanocomposite and its application as a cysteine / homocysteine colorimetric and fluorescence detector

ŠIMŠÍKOVÁ, M. ČECHAL, J. ZORKOVSKÁ, A. ANTALÍK, M. ŠIKOLA, T.

Original Title

Preparation of CuO/ZnO nanocomposite and its application as a cysteine / homocysteine colorimetric and fluorescence detector

Type

journal article in Web of Science

Language

English

Original Abstract

Cysteine and homocysteine play a crucial role in many biological functions but abnormal levels of these amino acids may lead to various forms of pathogenesis. Therefore, selective and easy-to-use methods for the detection of cysteine and homocysteine are essential for the early diagnosis of developing diseases. In this paper we report on a rapid, straightforward and highly selective method for the detection of cysteine (Cys) and homocysteine (Hcy) which uses a CuO-ZnO nanocomposite as a dual colorimetric and fluorometric assay. The presence of Cys and Hcy in a solution of these nanorods (NRs) induces a change in its color from light blue to dark grey which is visible to the naked eye. This is accompanied by a blue shift in the absorption spectra from 725 nm to 650 nm and a decrease in the intensity of CuO-ZnO nanocomposite emission. These changes are ascribed to the reduction of Cu(II) to Cu(0), and the oxidation of cysteine (homocysteine) and subsequent formation of the disulfide bond. This novel assay method does not respond to any other amino-acid which is present in living organisms; therefore the selective determination of cysteine (homocysteine) with a lower analyte limit of 40 microL can be carried out in aqueous solutions without the need for any sophisticated instrumentation, fluorophore molecules or complicated procedures.

Keywords

CuO/ZnO nanorods; Colorimetric sensor; Fluorescence; Cysteine; Homocysteine; Zero-valent copper

Authors

ŠIMŠÍKOVÁ, M.; ČECHAL, J.; ZORKOVSKÁ, A.; ANTALÍK, M.; ŠIKOLA, T.

RIV year

2014

Released

28. 11. 2014

ISBN

0927-7765

Periodical

Colloids and Surfaces B: Biointerfaces

Year of study

123

Number

1

State

Kingdom of the Netherlands

Pages from

951

Pages to

958

Pages count

8

BibTex

@article{BUT111089,
  author="Michaela {Šimšíková} and Jan {Čechal} and Anna {Zorkovská} and Marián {Antalík} and Tomáš {Šikola}",
  title="Preparation of CuO/ZnO nanocomposite and its application as a cysteine / homocysteine colorimetric and fluorescence detector",
  journal="Colloids and Surfaces B: Biointerfaces",
  year="2014",
  volume="123",
  number="1",
  pages="951--958",
  doi="10.1016/j.colsurfb.2014.10.051",
  issn="0927-7765"
}