Publication detail

Use of green fluorescent proteins for in vitro biosensing.

HEGER, Z. ZÍTKA, O. FOHLEROVÁ, Z. MERLOS RODRIGO, M. HUBÁLEK, J. KIZEK, R. ADAM, V.

Original Title

Use of green fluorescent proteins for in vitro biosensing.

Type

journal article in Web of Science

Language

English

Original Abstract

Due to the considerable stability of green fluorescent proteins and their capacity to be readily permutated or mutated, they may be exploited in multiple ways to enhance the functionality of in vitro biosensors. Many possibilities, such as the formation of chimeras with other proteins or antibodies, as well as Förster resonance emission transfer performance, may be used for the highly sensitive and specific detection of the target molecules. This review considers the great potential of green fluorescent proteins as the fluorescent probing or recognition biomolecule in various in vitro biosensors applications, as well as obstacles associated with their use.

Keywords

Aequorea victoria; biosensor; chimera; emission; Förster resonance emission transfer

Authors

HEGER, Z.; ZÍTKA, O.; FOHLEROVÁ, Z.; MERLOS RODRIGO, M.; HUBÁLEK, J.; KIZEK, R.; ADAM, V.

RIV year

2015

Released

1. 1. 2015

Publisher

Chemical Papers

ISBN

0366-6352

Periodical

Chemical Papers

Year of study

69

Number

1

State

Slovak Republic

Pages from

54

Pages to

61

Pages count

8

BibTex

@article{BUT112066,
  author="Zbyněk {Heger} and Ondřej {Zítka} and Zdenka {Fohlerová} and Miguel Ángel {Merlos Rodrigo} and Jaromír {Hubálek} and René {Kizek} and Vojtěch {Adam}",
  title="Use of green fluorescent proteins for in vitro biosensing.",
  journal="Chemical Papers",
  year="2015",
  volume="69",
  number="1",
  pages="54--61",
  doi="10.2478/s11696-014-0588-9",
  issn="0366-6352"
}