Publication detail

Paramagnetic Particles and PNA Probe for Automated Separation and Electrochemical Detection of Influenza

KREJČOVÁ, L. NGUYEN, H. HYNEK, D. GURÁŇ, R. ADAM, V. KIZEK, R.

Original Title

Paramagnetic Particles and PNA Probe for Automated Separation and Electrochemical Detection of Influenza

Type

journal article in Web of Science

Language

English

Original Abstract

Considerable efforts have been devoted to the development of rapid and sensitive methods allowing the detection of viral nucleic acid. We herein describe an assay for identification of a specific influenza sequence. The suggested method was based on isolation using paramagnetic particles coupled with electrochemical detection of isolated product. Peptide nucleic acid (PNA) was used as a probe for hybridization and identification of the influenza-derived specific sequence. The use of PNA can show numerous benefits: PNA probe is not degradable by enzymes and the duplex of PNA with RNA/DNA is more thermostable and more resistant to pH changes than DNA/DNA or RNA/ RNA duplexes. This PNA probe assay can be applied as a magnetically guidable tool for detection of DNA/RNA samples under different conditions.

Keywords

Protein nucleic acid, Biosensor, Magneticparticle- based separation, Voltammetry

Authors

KREJČOVÁ, L.; NGUYEN, H.; HYNEK, D.; GURÁŇ, R.; ADAM, V.; KIZEK, R.

RIV year

2014

Released

1. 11. 2014

ISBN

0009-5893

Periodical

CHROMATOGRAPHIA

Year of study

77

Number

21-22

State

Federal Republic of Germany

Pages from

1425

Pages to

1432

Pages count

8

BibTex

@article{BUT112354,
  author="Ludmila {Krejčová} and Hoai Viet {Nguyen} and David {Hynek} and Roman {Guráň} and Vojtěch {Adam} and René {Kizek}",
  title="Paramagnetic Particles and PNA Probe for Automated Separation and Electrochemical Detection of Influenza",
  journal="CHROMATOGRAPHIA",
  year="2014",
  volume="77",
  number="21-22",
  pages="1425--1432",
  doi="10.1007/s10337-014-2737-2",
  issn="0009-5893"
}