Hyaluronan-cetyltrimethylammonium bromide interactions in aqueous and physiological solution studied by fluorescence techniques

conference paper

English

The aim of this work was to study systems composed of negatively charged polyelectrolyte – hyaluronan and positively charged surfactant – cetyltrimethylammonium bromide (CTAB), which could be potential used for targeted drug delivery. Two concentration series was mixed – in an aqueous solution and in 0.15 mol l-1 sodium chloride (simply physiological solution). In the series, the concentration of hyaluronan was constant and the concentration of CTAB (well below the critical micelle concentration in water solution) was increased. The interactions between these components were studied in terms of the formation of hydrophobic domains, which were important for solubilization of molecules of hydrophobic drugs. The intensity of fluorescence, steady-state anisotropy and lifetime of the samples were measured. Perylene was chosen as fluorescent probe due to its hydrophobicity. It was found that in aqueous solution, small hydrophobic domains linked to hyaluronan chains are formed. Then an increasing concentration of CTAB leads to phase separation and formation of the condensed phase. Addition of sodium chloride to the samples leads to the reorganization of this system – the condensed phase is not created and probably free micelles (possibly mixture of micelle linked to hyaluronan chain with free micelle) are formed in the solution.

Fluorescence anisotropy, interaction, perylene, minimicelles, hydrophobic domains, CAC, critical aggregation concentration

HOLÍNKOVÁ, P.; MRAVEC, F.; PEKAŘ, M.

2014

4. 12. 2014

Brno

978-80-214-5078-3

Chemie je život 2014 - Sborník příspěvků

1

1

229

234

6