Publication detail

Identification of probiotic bacteria in foods and other complex matrices

ŠPANOVÁ, A.,TRACHTOVÁ, Š., ZOVČÁKOVÁ, M., ČAKAJDOVÁ, M., VAŠÍČEK, R., OLIVA, J., HORÁK, D., RITTICH, B.

Original Title

Identification of probiotic bacteria in foods and other complex matrices

Type

abstract

Language

English

Original Abstract

Different types of magnetic non-porous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) - P(HEMA-co-GMA) microspheres, poly(2-hydroxyethyl methacrylate-co-etylene dimethacrylate - P(HEMA-co-EDMA) and poly(glycidylmethacrylate) - (PGMA) covered by carboxyl groups were tested for the DNA isolation from crude cell lysates of probiotic dairy products, fermented meat products (probiotic salami), food additives, feces etc. These types of products represent a matrix characterized by a complex composition including the presence of inhibitors that affect the course of PCR. Isolated DNAs were without PCR inhibitors which was demonstrated using PCR and real-time PCR. Probiotic species were identified in accordance with the declaration of producers usually.

Keywords

probiotic bacteria; Lactobacillus; magnetic microparticles; foods

Authors

ŠPANOVÁ, A.,TRACHTOVÁ, Š., ZOVČÁKOVÁ, M., ČAKAJDOVÁ, M., VAŠÍČEK, R., OLIVA, J., HORÁK, D., RITTICH, B.

Released

24. 5. 2015

Publisher

Institute of Immunology and Experimental Therapy Polish Academy of Science

Location

Bielawa, Polsko

ISBN

978-83-928488-4-4

Book

2nd Polish-Czech Probiotic Conference: Microbiology, Immunology and Alergy

Edition number

1

Pages from

41

Pages to

41

Pages count

1

BibTex

@misc{BUT114815,
  author="Bohuslav {Rittich} and Alena {Španová} and Štěpánka {Trachtová} and Monika {Zovčáková} and Martina {Čakajdová} and Roman {Vašíček} and Jan {Oliva} and Daniel {Horák}",
  title="Identification of probiotic bacteria in foods and other complex matrices",
  booktitle="2nd Polish-Czech Probiotic Conference: Microbiology, Immunology and Alergy",
  year="2015",
  edition="1",
  pages="41--41",
  publisher="Institute of Immunology and Experimental Therapy Polish Academy of Science",
  address="Bielawa, Polsko",
  isbn="978-83-928488-4-4",
  note="abstract"
}