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ŠPANOVÁ, A.,TRACHTOVÁ, Š., ZOVČÁKOVÁ, M., ČAKAJDOVÁ, M., VAŠÍČEK, R., OLIVA, J., HORÁK, D., RITTICH, B.
Original Title
Identification of probiotic bacteria in foods and other complex matrices
Type
abstract
Language
English
Original Abstract
Different types of magnetic non-porous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) - P(HEMA-co-GMA) microspheres, poly(2-hydroxyethyl methacrylate-co-etylene dimethacrylate - P(HEMA-co-EDMA) and poly(glycidylmethacrylate) - (PGMA) covered by carboxyl groups were tested for the DNA isolation from crude cell lysates of probiotic dairy products, fermented meat products (probiotic salami), food additives, feces etc. These types of products represent a matrix characterized by a complex composition including the presence of inhibitors that affect the course of PCR. Isolated DNAs were without PCR inhibitors which was demonstrated using PCR and real-time PCR. Probiotic species were identified in accordance with the declaration of producers usually.
Keywords
probiotic bacteria; Lactobacillus; magnetic microparticles; foods
Authors
Released
24. 5. 2015
Publisher
Institute of Immunology and Experimental Therapy Polish Academy of Science
Location
Bielawa, Polsko
ISBN
978-83-928488-4-4
Book
2nd Polish-Czech Probiotic Conference: Microbiology, Immunology and Alergy
Edition number
1
Pages from
41
Pages to
Pages count
BibTex
@misc{BUT114815, author="Bohuslav {Rittich} and Alena {Španová} and Štěpánka {Trachtová} and Monika {Zovčáková} and Martina {Čakajdová} and Roman {Vašíček} and Jan {Oliva} and Daniel {Horák}", title="Identification of probiotic bacteria in foods and other complex matrices", booktitle="2nd Polish-Czech Probiotic Conference: Microbiology, Immunology and Alergy", year="2015", edition="1", pages="41--41", publisher="Institute of Immunology and Experimental Therapy Polish Academy of Science", address="Bielawa, Polsko", isbn="978-83-928488-4-4", note="abstract" }