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ŠKARKOVÁ, P. VÁVROVÁ, M. ROMIH, T. JEMEC, A. DROBNE, D.
Original Title
Hazardous potential of gold nanoparticles identified by in vivo assay on Arthropod (Porcellio scaber, Isopoda, Crustacea)
Type
conference proceedings
Language
English
Original Abstract
In the presented study, we assessed the influence of gold nanoparticles (AuNPs) and AuCl3 on different biological endpoints of the model organism Porcellio scaber. By employing a cell membrane integrity assay, we tested whether gold compounds have the potential to cell membrane destabilization, which could lead to direct entry of AuNPs into hepatopancreatic cells. We coupled this technique with AAS measurements, to corroborate whether AuNPs internalization or Au+ assimilation actually occurred. Furthermore, we tested the adverse biological effects of AuNPs and AuCl3 on Porcellio scaber, detectable via morphometric characteristics of the hepatopancreatic epithelium and standard toxicological parameters (body mass change, food consumption and mortality). We were interested by possible avoidance behavior in 14 days of exposure to different sources of food at the same time. In this experiment avoidance behavior was not observed. The mass of consumed leaves were almost identical from both pieces (control and contaminated) for tested concentrations compounds Au NPs and AuCl3.
Keywords
toxicity, gold nanopartciles, cells, tissues, Porcelio scaber
Authors
ŠKARKOVÁ, P.; VÁVROVÁ, M.; ROMIH, T.; JEMEC, A.; DROBNE, D.
Released
26. 9. 2015
Pages from
242
Pages to
Pages count
1
URL
http://www.mesaep.org/
BibTex
@proceedings{BUT117885, editor="ŠKARKOVÁ, P. and VÁVROVÁ, M. and ROMIH, T. and JEMEC, A. and DROBNE, D.", title="Hazardous potential of gold nanoparticles identified by in vivo assay on Arthropod (Porcellio scaber, Isopoda, Crustacea)", year="2015", pages="242--242", url="http://www.mesaep.org/" }