Publication detail

A 3D Microfluidic Chip for Electrochemical Detection of Hydrolysed Nucleic Bases by a Modified Glassy Carbon Electrode

VLACHOVÁ, J. TMEJOVÁ, K. KOPEL, P. KORABÍK, M. ZÍTKA, J. HYNEK, D. KYNICKÝ, J. ADAM, V. KIZEK, R.

Original Title

A 3D Microfluidic Chip for Electrochemical Detection of Hydrolysed Nucleic Bases by a Modified Glassy Carbon Electrode

Type

journal article in Web of Science

Language

English

Original Abstract

Modification of carbon materials, especially graphene-based materials, has wide applications in electrochemical detection such as electrochemical lab-on-chip devices. A glassy carbon electrode (GCE) modified with chemically alternated graphene oxide was used as a working electrode (glassy carbon modified by graphene oxide with sulphur containing compounds and Nafion) for detection of nucleobases in hydrolysed samples (HCl pH = 2.9, 100 °C, 1 h, neutralization by NaOH). It was found out that modification, especially with trithiocyanuric acid, increased the sensitivity of detection in comparison with pure GCE. All processes were finally implemented in a microfluidic chip formed with a 3D printer by fused deposition modelling technology. As a material for chip fabrication, acrylonitrile butadiene styrene was chosen because of its mechanical and chemical stability. The chip contained the one chamber for the hydrolysis of the nucleic acid and another for the electrochemical detection by the modified GCE. This chamber was fabricated to allow for replacement of the GCE.

Keywords

REDUCED GRAPHENE OXIDE; TRITHIOCYANURIC ACID; 2,4,6-TRIMERCAPTO-1,3,5-TRIAZINE TMT; FUNCTIONALIZED GRAPHENE; COMPLEXES; CHEMISTRY; SALT; 1,3,5-TRIAZINE-2,4,6-TRITHIOL; TRIMERCAPTOTRIAZINE; INHIBITION

Authors

VLACHOVÁ, J.; TMEJOVÁ, K.; KOPEL, P.; KORABÍK, M.; ZÍTKA, J.; HYNEK, D.; KYNICKÝ, J.; ADAM, V.; KIZEK, R.

RIV year

2015

Released

1. 2. 2015

Publisher

MDPI AG, POSTFACH, CH-4005 BASEL, SWITZERLAND

Location

BASEL

ISBN

1424-8220

Periodical

SENSORS

Year of study

15

Number

2

State

Swiss Confederation

Pages from

2438

Pages to

2452

Pages count

15

URL

http://www.mdpi.com/1424-8220/15/2/2438

Full text in the Digital Library

http://hdl.handle.net/11012/63725

BibTex

@article{BUT123331,
  author="Jana {Vlachová} and Kateřina {Tmejová} and Pavel {Kopel} and Michal {Korabík} and Jan {Zítka} and David {Hynek} and Jindřich {Kynický} and Vojtěch {Adam} and René {Kizek}",
  title="A 3D Microfluidic Chip for Electrochemical Detection of Hydrolysed Nucleic Bases by a Modified Glassy Carbon Electrode",
  journal="SENSORS",
  year="2015",
  volume="15",
  number="2",
  pages="2438--2452",
  doi="10.3390/s150202438",
  issn="1424-8220",
  url="http://www.mdpi.com/1424-8220/15/2/2438"
}