Publication detail

Imaging of Norway spruce early somatic embryos with the ESEM, Cryo-SEM and laser scanning microscope

NEDĚLA, V. HŘIB, J. HAVEL, L. HUDEC, J. RUNŠTUK, J.

Original Title

Imaging of Norway spruce early somatic embryos with the ESEM, Cryo-SEM and laser scanning microscope

Type

journal article - other

Language

English

Original Abstract

This article describes the surface structure of Norway spruce early somatic embryos (ESEs) as a typical culture with asynchronous development. The microstructure of extracellular matrix covering ESEs were observed using the environmental scanning electron microscope as a primary tool and using the scanning electron microscope with cryo attachment and laser electron microscope as a complementary tool allowing our results to be proven independently. The fresh samples were observed in conditions of the air environment of the environmental scanning electron microscope (ESEM) with the pressure from 550 Pa to 690 Pa and the low temperature of the sample from −18 °C to −22 °C. The samples were studied using two different types of detector to allow studying either the thin surface structure or material composition. The scanning electron microscope with cryo attachment was used for imaging frozen extracellular matrix microstructure with higher resolution. The combination of both electron microscopy methods was suitable for observation of “native” plant samples, allowing correct evaluation of our results, free of error and artifacts.

Keywords

ESEM; Cryo-SEM; Bright field/dark field microscopy; Extracellular matrix; Picea abies; Somatic embryogenesis

Authors

NEDĚLA, V.; HŘIB, J.; HAVEL, L.; HUDEC, J.; RUNŠTUK, J.

Released

2. 3. 2016

Publisher

Elsevier

ISBN

0968-4328

Periodical

Micron

Year of study

2016

Number

84

State

United Kingdom of Great Britain and Northern Ireland

Pages from

67

Pages to

71

Pages count

5

URL

http://www.sciencedirect.com/science/article/pii/S0968432816300208

BibTex

@article{BUT123955,
  author="Vilém {Neděla} and Jiří {Hřib} and Ladislav {Havel} and Jiří {Hudec} and Jiří {Runštuk}",
  title="Imaging of Norway spruce early somatic embryos with the ESEM, Cryo-SEM and laser scanning microscope",
  journal="Micron",
  year="2016",
  volume="2016",
  number="84",
  pages="67--71",
  doi="10.1016/j.micron.2016.02.011",
  issn="0968-4328",
  url="http://www.sciencedirect.com/science/article/pii/S0968432816300208"
}