Publication detail

Antiviral activity of fullerene C60 nanocrystals modified with derivatives of anionic antimicrobial peptide maximin H5

DOSTÁLOVÁ, S. MOULICK, A. MILOSAVLJEVIĆ, V. GURÁŇ, R. KOMÍNKOVÁ, M. ČÍHALOVÁ, K. HEGER, Z. BLAŽKOVÁ, L. KOPEL, P. HYNEK, D. VACULOVIČOVÁ, M. ADAM, V. KIZEK, R.

Original Title

Antiviral activity of fullerene C60 nanocrystals modified with derivatives of anionic antimicrobial peptide maximin H5

Type

journal article in Web of Science

Language

English

Original Abstract

Many active antiviral substances come from natural sources. In this way, peptides, isolated from Asian toad Bombina maxima, called maximins, are very promising. Most of them have good antimicrobial activity; however, derivatives of anionic 20 amino acids-long maximin H5 show also promising antiviral activity. The effect can be enhanced by binding to suitable nanocarriers such as fullerenes. In the present study, six mutants of maximin H5 were designed where aspartic acid at position 11 was replaced by asparagine, histidine, tyrosine, alanine, glycine, or valine. The binding yield of each peptide to fullerene C60 nanocrystals was studied by derivatization with fluorescent reagent fluorescamine. The antiviral activity of these peptides and peptides bound to fullerene C60 nanocrystals was studied using bacteriophage k as a model virus. All of the designed peptides had higher antiviral activity compared to maximin H5. The highest antiviral activity was observed in case of maximin variants H5N, H5V, or H5Y. Moreover, the antiviral activity was dependent on the amount of peptide bound on the surface of fullerene C60 nanocrystals, which was enhanced by trimesic acid (benzene-1,3,5-tricarboxylic acid) treated fullerene C60 nanocrystals.

Keywords

Carbon nanomaterials  Maximin H5  Nanocarrier  Nanomedicine  Viral infections

Authors

DOSTÁLOVÁ, S.; MOULICK, A.; MILOSAVLJEVIĆ, V.; GURÁŇ, R.; KOMÍNKOVÁ, M.; ČÍHALOVÁ, K.; HEGER, Z.; BLAŽKOVÁ, L.; KOPEL, P.; HYNEK, D.; VACULOVIČOVÁ, M.; ADAM, V.; KIZEK, R.

Released

1. 5. 2016

ISBN

1434-4475

Periodical

Monatshefte für Chemie

Year of study

147

Number

5

State

Republic of Austria

Pages from

905

Pages to

918

Pages count

14

BibTex

@article{BUT128500,
  author="Simona {Dostálová} and Amitava {Moulick} and Vedran {Milosavljević} and Roman {Guráň} and Markéta {Komínková} and Kristýna {Číhalová} and Zbyněk {Heger} and Lucie {Blažková} and Pavel {Kopel} and David {Hynek} and Markéta {Vaculovičová} and Vojtěch {Adam} and René {Kizek}",
  title="Antiviral activity of fullerene C60 nanocrystals modified with derivatives of anionic antimicrobial peptide maximin H5",
  journal="Monatshefte für Chemie",
  year="2016",
  volume="147",
  number="5",
  pages="905--918",
  doi="10.1007/s00706-016-1675-0",
  issn="1434-4475"
}