Publication detail

The Zinc-Schiff Base-Novicidin Complex as a Potential Prostate Cancer Therapy

MILOSAVLJEVIĆ, V. HADDAD, Y. MERLOS RODRIGO, M. MOULICK, A. POLANSKÁ, H. HYNEK, D. HEGER, Z. KOPEL, P. ADAM, V.

Original Title

The Zinc-Schiff Base-Novicidin Complex as a Potential Prostate Cancer Therapy

Type

journal article in Web of Science

Language

English

Original Abstract

Prostate cancer cells control energy metabolism by chelating intracellular zinc. Thus, zinc delivery has been a popular therapeutic approach for prostate cancer. Here, we propose the use of the membrane-penetrating peptide Novicidin connected to zinc-Schiff base as a carrier vehicle for the delivery of zinc to prostate cells. Mass spectrometry, electrochemistry and spectrophotometry confirmed the formation/stability of this complex and provided insight regarding the availability of zinc for complex interactions. This delivery system showed minor toxicity in normal PNT1A cells and high potency towards PC3 tumor cells. The complex preferentially penetrated PC3 tumor cells in contrast to confinement to the membranes of PNT1A. Furthermore, zinc uptake was confirmed in both cell lines. Molecular analysis was used to confirm the activation of zinc stress (e.g., ZnT-1) and apoptosis (e.g., CASP-1). Our results strongly suggest that the zinc-Schiff base-Novicidin complex has great potential as a novel anticancer drug.

Keywords

Zinc-Schiff Base-Novicidin Complex, Prostate Cancer

Authors

MILOSAVLJEVIĆ, V.; HADDAD, Y.; MERLOS RODRIGO, M.; MOULICK, A.; POLANSKÁ, H.; HYNEK, D.; HEGER, Z.; KOPEL, P.; ADAM, V.

Released

11. 10. 2016

Publisher

PLOS

ISBN

1932-6203

Periodical

PLOS ONE

Year of study

11

Number

10

State

United States of America

Pages from

1

Pages to

19

Pages count

19

URL

http://europepmc.org/articles/PMC5058503?pdf=render

Full text in the Digital Library

http://hdl.handle.net/11012/69382

BibTex

@article{BUT129691,
  author="Vedran {Milosavljević} and Yazan Abdulmajeed Eyadh {Haddad} and Miguel Ángel {Merlos Rodrigo} and Amitava {Moulick} and Hana {Polanská} and David {Hynek} and Zbyněk {Heger} and Pavel {Kopel} and Vojtěch {Adam}",
  title="The Zinc-Schiff Base-Novicidin Complex as a Potential Prostate Cancer Therapy",
  journal="PLOS ONE",
  year="2016",
  volume="11",
  number="10",
  pages="1--19",
  doi="10.1371/journal.pone.0163983",
  issn="1932-6203",
  url="http://europepmc.org/articles/PMC5058503?pdf=render"
}