Publication detail

Fully automated two-step assay for detection of metallothioneinthrough magnetic isolation using functionalized -Fe2O3particles

MERLOS RODRIGO, M. KREJČOVÁ, L. KUDR, J. CERNEI, N. KOPEL, P. RICHTERA, L. MOULICK, A. HYNEK, D. ADAM, V. STIBOROVÁ, M. ECKSCHLAGER, T. HEGER, Z. ZÍTKA, O.

Original Title

Fully automated two-step assay for detection of metallothioneinthrough magnetic isolation using functionalized -Fe2O3particles

Type

journal article in Web of Science

Language

English

Original Abstract

tMetallothioneins (MTs) are involved in heavy metal detoxification in a wide range of living organisms.Currently, it is well known that MTs play substantial role in many pathophysiological processes, includingcarcinogenesis, and they can serve as diagnostic biomarkers. In order to increase the applicability of MTin cancer diagnostics, an easy-to-use and rapid method for its detection is required. Hence, the aim of thisstudy was to develop a fully automated and high-throughput assay for the estimation of MT levels. Here,we report the optimal conditions for the isolation of MTs from rabbit liver and their characterizationusing MALDI-TOF MS. In addition, we described a two-step assay, which started with an isolation ofthe protein using functionalized paramagnetic particles and finished with their electrochemical analysis.The designed easy-to-use, cost-effective, error-free and fully automated procedure for the isolation ofMT coupled with a simple analytical detection method can provide a prototype for the construction of adiagnostic instrument, which would be appropriate for the monitoring of carcinogenesis or MT-relatedchemoresistance of tumors.

Keywords

Automation, Biomarkers, Magnetic isolation, MALDI-TOF MS, Metallothioneina

Authors

MERLOS RODRIGO, M.; KREJČOVÁ, L.; KUDR, J.; CERNEI, N.; KOPEL, P.; RICHTERA, L.; MOULICK, A.; HYNEK, D.; ADAM, V.; STIBOROVÁ, M.; ECKSCHLAGER, T.; HEGER, Z.; ZÍTKA, O.

Released

15. 12. 2016

ISBN

1570-0232

Periodical

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Year of study

1039

Number

1

State

Kingdom of the Netherlands

Pages from

17

Pages to

27

Pages count

11

BibTex

@article{BUT131500,
  author="Miguel Ángel {Merlos Rodrigo} and Ludmila {Krejčová} and Jiří {Kudr} and Natalia Vladimirovna {Cernei} and Pavel {Kopel} and Lukáš {Richtera} and Amitava {Moulick} and David {Hynek} and Vojtěch {Adam} and Marie {Stiborová} and Tomáš {Eckschlager} and Zbyněk {Heger} and Ondřej {Zítka}",
  title="Fully automated two-step assay for detection of metallothioneinthrough magnetic isolation using functionalized -Fe2O3particles",
  journal="JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES",
  year="2016",
  volume="1039",
  number="1",
  pages="17--27",
  doi="10.1016/j.jchromb.2016.10.018",
  issn="1570-0232"
}