Publication detail

Determination of s-adenosylmethionine and s-adenosylhomocysteine in two prostatic adenocarcinoma cell lines

GURÁŇ, R. POMPEIANO VANÍČKOVÁ, L. LACKOVÁ, Z. BUCHTELOVÁ, H. MICHÁLEK, P. HEGER, Z. ZÍTKA, O.

Original Title

Determination of s-adenosylmethionine and s-adenosylhomocysteine in two prostatic adenocarcinoma cell lines

Type

conference paper

Language

English

Original Abstract

Studies of processes leading to cancer tumour progression are one of the major foci of current cancer research. Therefore, it is important to shade the light on the metabolic pathways involved in cancerogenesis. In the present study we have chosen as a model two prostatic adenocarcinoma cell lines (PC-3 and LNCaP) for the comparison of the metabolites involved in tumour growth. Applying the HPLC-MS method, we determined and compared the concentration levels of S-(5’-adenosyl)-Lhomocysteine (SAH) and S-(5’-adenosyl)-L-methionine (SAM), that are involved in the tumour growth processes. SAM/SAH ratio, a methylation index describing the aggressiveness of tumour cells, was determined here. The methylation index was 0.4 and 1.2 for PC-3 and LNCaP cells, respectively, pointing out that the PC-3 cells are more aggressive than LNCaP cells. This finding is of great importance for our following studies

Keywords

SAM; SAH; methylation index; PC-3; LNCaP; metallothionein

Authors

GURÁŇ, R.; POMPEIANO VANÍČKOVÁ, L.; LACKOVÁ, Z.; BUCHTELOVÁ, H.; MICHÁLEK, P.; HEGER, Z.; ZÍTKA, O.

Released

12. 12. 2016

Publisher

Mendel University in Brno

Location

Brno, Czech Republic

ISBN

978-80-7509-443-8

Book

MendelNet2016

Pages from

972

Pages to

977

Pages count

6

BibTex

@inproceedings{BUT132913,
  author="Roman {Guráň} and Lucie {Pompeiano Vaníčková} and Zuzana {Lacková} and Hana {Michálková} and Petr {Michálek} and Zbyněk {Heger} and Ondřej {Zítka}",
  title="Determination of s-adenosylmethionine and s-adenosylhomocysteine in two prostatic adenocarcinoma cell lines",
  booktitle="MendelNet2016",
  year="2016",
  pages="972--977",
  publisher="Mendel University in Brno",
  address="Brno, Czech Republic",
  isbn="978-80-7509-443-8"
}