Publication detail

Evaluation of poly(ethylene glycol)-coated monodispersed magnetic poly(2-hydroxyethyl methacrylate) and poly(glycidyl methacrylate) microspheres by PCR

HORÁK, D. HLÍDKOVÁ, H. TRACHTOVÁ, Š. ŠLOUF, M. RITTICH, B. ŠPANOVÁ, A.

Original Title

Evaluation of poly(ethylene glycol)-coated monodispersed magnetic poly(2-hydroxyethyl methacrylate) and poly(glycidyl methacrylate) microspheres by PCR

Type

journal article in Web of Science

Language

English

Original Abstract

New monodisperse magnetic poly(2-hydroxyethyl methacrylate) and poly(glycidyl methacrylate) microspheres were synthesized, coated with poly(ethylene glycol) and thoroughly characterized using several methods, such as scanning and transmission electron microscopy equipped with energy-dispersive X-ray spectroscopy (EDX), elemental analysis, ATR FT-IR and atomic absorption spectroscopy. The effect of different coatings of the microspheres on the DNA amplification was finally investigated using a real-time polymerase chain reaction (qPCR). The particles with a relatively high density of poly(ethylene glycol) and a low amount of carboxyl groups on the surface appeared suitable for DNA isolation.

Keywords

Magnetic microspheres; Poly(ethylene glycol); Real-time PCR; DNA isolation

Authors

HORÁK, D.; HLÍDKOVÁ, H.; TRACHTOVÁ, Š.; ŠLOUF, M.; RITTICH, B.; ŠPANOVÁ, A.

RIV year

2015

Released

26. 4. 2015

Publisher

Elsevier

Location

Spojené království Velké Británie a Severního Irska

ISBN

0014-3057

Periodical

European Polymer Journal

Year of study

68

Number

1

State

United Kingdom of Great Britain and Northern Ireland

Pages from

687

Pages to

696

Pages count

10

URL

http://ac.els-cdn.com/S0014305715001718/1-s2.0-S0014305715001718-main.pdf?_tid=d8af3582-09ed-11e5-adc1-00000aab0f02&acdnat=1433335465_825a170c92c2f3d7ffed38fe9581d0d0

BibTex

@article{BUT133347,
  author="Daniel {Horák} and Helena {Hlídková} and Štěpánka {Trachtová} and Miloslav {Šlouf} and Bohuslav {Rittich} and Alena {Španová}",
  title="Evaluation of poly(ethylene glycol)-coated monodispersed magnetic poly(2-hydroxyethyl methacrylate) and poly(glycidyl methacrylate) microspheres by PCR",
  journal="European Polymer Journal",
  year="2015",
  volume="68",
  number="1",
  pages="687--696",
  doi="10.1016/j.eurpolymj.2015.03.036",
  issn="0014-3057",
  url="http://ac.els-cdn.com/S0014305715001718/1-s2.0-S0014305715001718-main.pdf?_tid=d8af3582-09ed-11e5-adc1-00000aab0f02&acdnat=1433335465_825a170c92c2f3d7ffed38fe9581d0d0"
}