Publication detail

Antibody-free detection of infectious bacteria using quantum dots-based barcode assay

ČÍHALOVÁ, K. HEGEROVÁ, D. JIMENEZ JIMENEZ, A. MILOSAVLJEVIĆ, V. KUDR, J. SKALIČKOVÁ, S. HYNEK, D. KOPEL, P. VACULOVIČOVÁ, M. ADAM, V.

Original Title

Antibody-free detection of infectious bacteria using quantum dots-based barcode assay

Type

journal article in Web of Science

Language

English

Original Abstract

Staphylococcus aureus, methicillin-resistant Staphylococcus aureus and Klebsiella pneumoniae are the mostrepresentative bacteria causing infectious diseases. Due to the increased application of antibiotics, thebacterial resistance is growing causing severe complications. Therefore, a sensitive determination of thesepathogens is crucial for effective treatment.The aim of this study was to design an effective method for multiplex detection of Staphylococcus aureus,methicillin-resistant Staphylococcus aureus and Klebsiella pneumoniae taking advantage from propertiesof magnetic particles as well as fluorescent nanoparticles (quantum dots). The method was able to detectas low concentrations of bacteria as 102CFU/mL using the bacteria-specific genes (fnbA, mecA and wcaG).

Keywords

Staphylococcus aureus; Methicillin-resistant; Staphylococcus aureus; Klebsiella pneumoniae; Magnetic particles; Barcode; Quantum dots

Authors

ČÍHALOVÁ, K.; HEGEROVÁ, D.; JIMENEZ JIMENEZ, A.; MILOSAVLJEVIĆ, V.; KUDR, J.; SKALIČKOVÁ, S.; HYNEK, D.; KOPEL, P.; VACULOVIČOVÁ, M.; ADAM, V.

Released

5. 2. 2017

ISBN

0731-7085

Periodical

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Year of study

134

Number

1

State

Kingdom of the Netherlands

Pages from

325

Pages to

332

Pages count

8

BibTex

@article{BUT133964,
  author="ČÍHALOVÁ, K. and HEGEROVÁ, D. and JIMENEZ JIMENEZ, A. and MILOSAVLJEVIĆ, V. and KUDR, J. and SKALIČKOVÁ, S. and HYNEK, D. and KOPEL, P. and VACULOVIČOVÁ, M. and ADAM, V.",
  title="Antibody-free detection of infectious bacteria using quantum dots-based barcode assay",
  journal="JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS",
  year="2017",
  volume="134",
  number="1",
  pages="325--332",
  doi="10.1016/j.jpba.2016.10.025",
  issn="0731-7085"
}