Publication detail

Three-dimensional fluorescence lifetime imaging in confocal microscopy of living cells

BAIAZITOVA, L. ČMIEL, V. SKOPALÍK, J. SVOBODA, O. PROVAZNÍK, I.

Original Title

Three-dimensional fluorescence lifetime imaging in confocal microscopy of living cells

Type

conference paper

Language

English

Original Abstract

Fluorescence lifetime imaging (FLIM) is a modern optical method which increases the potential of standard microscopy. This paper shows the possibilities of extended fluorescence lifetime evaluation and imaging in studying three-dimensional structures such as compartments of living cells with different fluorescence lifetimes. The method for quasi-FLIM image calculation is presented and image processing steps useful for biological experiments are suggested.

Keywords

Fluorescence lifetime; FLIM; cardiomyocyte; stromal cell; image segmentation

Authors

BAIAZITOVA, L.; ČMIEL, V.; SKOPALÍK, J.; SVOBODA, O.; PROVAZNÍK, I.

Released

28. 8. 2017

Location

Kos island, Greece

ISBN

978-0-9928626-7-1

Book

2017 25th European Signal Processing Conference (EUSIPCO)

ISBN

2219-5491

Periodical

European Signal Processing Conference EUSIPCO

State

United States of America

Pages from

439

Pages to

443

Pages count

5

URL

https://ieeexplore.ieee.org/document/8081245

BibTex

@inproceedings{BUT138959,
  author="Larisa {Chmelíková} and Vratislav {Čmiel} and Josef {Skopalík} and Ondřej {Svoboda} and Valentine {Provazník}",
  title="Three-dimensional fluorescence lifetime imaging in confocal microscopy of living cells",
  booktitle="2017 25th European Signal Processing Conference (EUSIPCO)",
  year="2017",
  journal="European Signal Processing Conference EUSIPCO",
  pages="439--443",
  address="Kos island, Greece",
  doi="10.23919/EUSIPCO.2017.8081245",
  isbn="978-0-9928626-7-1",
  issn="2219-5491",
  url="https://ieeexplore.ieee.org/document/8081245"
}