Publication detail

Bioconjugation of upconversion nanoparticle characterized by capillary electrophoresis

VANĚČKOVÁ, T. ŠMERKOVÁ, K. ZÍTKA, J. HYNEK, D. VACULOVIČOVÁ, M. NEJDL, L.

Original Title

Bioconjugation of upconversion nanoparticle characterized by capillary electrophoresis

Type

abstract

Language

English

Original Abstract

Upconversion nanoparticles (UCNPs) are an emerging class of optical materials with high potential in bioimaging due to practically no background signal and high penetration depth. Their excellent optical properties and easy surface functionalization make them perfect for conjugation with targeting ligands. In this work, capillary electrophoretic (CE) method with laserinduced fluorescence detection was used to investigate the behavior of carboxy-silica-UCNPs. Folic acid, targeting folate receptor overexpressed by wide variety of cancer cells, was used for illustrative purposes and assessed by CE under optimized conditions. Despite the numerous advantages of CE, this is the first time that CE was employed for characterization of UCNPs and their bioconjugates. The separation conditions were optimized including the background electrolyte concentration and pH. The optimized electrolyte was 20 mM borate buffer with pH 8.

Keywords

Upconversion nanoparticles (UCNPs); Capillary electrophoresis

Authors

VANĚČKOVÁ, T.; ŠMERKOVÁ, K.; ZÍTKA, J.; HYNEK, D.; VACULOVIČOVÁ, M.; NEJDL, L.

Released

18. 5. 2018

ISBN

978-88-941816-1-6

Book

42nd International Symposium on Capillary Chromatography and 15th GC×GC Symposium ABSTRACT BOOK

Pages from

266

Pages to

266

Pages count

1

BibTex

@misc{BUT147920,
  author="Tereza {Vaněčková} and Kristýna {Šmerková} and Jan {Zítka} and David {Hynek} and Markéta {Vaculovičová} and Lukáš {Nejdl}",
  title="Bioconjugation of upconversion nanoparticle characterized by capillary electrophoresis",
  booktitle="42nd International Symposium on Capillary Chromatography and 15th GC×GC Symposium
ABSTRACT BOOK",
  year="2018",
  pages="266--266",
  isbn="978-88-941816-1-6",
  note="abstract"
}